报告题目：IncHI2-Mediated Multiple Antimicrobial Resistance Dissemination in Salmonella enterica

报告人：施春雷

时  间： 2018年06月15日 星期五上午10:00-11:00

地  点： 闵行校区生物药学楼树华多功能厅（800号）

主持人：  杨广宇 研究员

报告人简介：

施春雷，上海交通大学农业与生物学院研究员。2006年在上海交通大学获生物医学工程（生物技术）博士学位，在美国康奈尔大学食品科学系从事博士后研究。致力于食品微生物性安全基础理论与控制技术的研究工作，重点开展了食源性致病菌菌膜形成分子机制基础研究，开发食源性致病菌快速检测和精准分型技术，并应用于微生物风险评估，首次提出了菌膜形成的抗氧胁迫协同调控模型。发表SCI论文47篇，主持国家重点研发计划项目一项。

报告摘要：

The widely usage of antimicrobials contributes to the increase in prevalence of antimicrobial-resistant Salmonella. And plasmid plays a critical role in horizontal transfer of antimicrobial-resistance markers in Salmonella. Here, we aimed to characterize plasmid profile responsible for antimicrobial-resistance markers and clarify genetic characteristics of antimicrobial-resistance plasmids. There are 44 Salmonella isolates were examined resistant to at least one antimicrobial with highest resistance against Ampicillin. These antimicrobial-resistant isolates were screened for plasmid-mediated quinolone resistance genes and β-lactamase resistance genes. The predominant plasmid replicon typing was IncHI2. After plasmid curing assay. The loss of IncHI2 plasmids was correlated with the loss of two groups of antimicrobial resistance genes alone or in combination. Meanwhile, the resultant IncHI2-positive isolates were tested harboring the three mobile genetic elements. After screening for antimicrobial susceptibility, 61 isolates were detected simultaneously harboring IncHI2 plasmid, IS26 and class 1 Integron, and 59 were confirmed as multidrug resistant, to Sulfisoxazole (100%), Ampicillin (90.16%), and Trimethoprim-Sulfamethoxole (90.16%). All these results warned us against the high risk of IncHI2-mediated multiple antimicrobial resistance dissemination in S. enterica.