报告题目：IncHI2-Mediated Multiple Antimicrobial Resistance Dissemination in Salmonella enterica
时 间： 2018年06月15日 星期五上午10:00-11:00
地 点： 闵行校区生物药学楼树华多功能厅（800号）
主持人： 杨广宇 研究员
The widely usage of antimicrobials contributes to the increase in prevalence of antimicrobial-resistant Salmonella. And plasmid plays a critical role in horizontal transfer of antimicrobial-resistance markers in Salmonella. Here, we aimed to characterize plasmid profile responsible for antimicrobial-resistance markers and clarify genetic characteristics of antimicrobial-resistance plasmids. There are 44 Salmonella isolates were examined resistant to at least one antimicrobial with highest resistance against Ampicillin. These antimicrobial-resistant isolates were screened for plasmid-mediated quinolone resistance genes and β-lactamase resistance genes. The predominant plasmid replicon typing was IncHI2. After plasmid curing assay. The loss of IncHI2 plasmids was correlated with the loss of two groups of antimicrobial resistance genes alone or in combination. Meanwhile, the resultant IncHI2-positive isolates were tested harboring the three mobile genetic elements. After screening for antimicrobial susceptibility, 61 isolates were detected simultaneously harboring IncHI2 plasmid, IS26 and class 1 Integron, and 59 were confirmed as multidrug resistant, to Sulfisoxazole (100%), Ampicillin (90.16%), and Trimethoprim-Sulfamethoxole (90.16%). All these results warned us against the high risk of IncHI2-mediated multiple antimicrobial resistance dissemination in S. enterica.