报告题目:Gut remediation: a novel approach to remediate food heavy metal contamination

报  告 人：李祥锴 教授     兰州大学生命科学学院；

报告时间：4月24日上午10:30-11:30

报告地点：闵行校区生物药学楼3#105会议室

联  系 人：张晓君 xjzhang68@sjtu.edu.cn
 

ABSTRACT

Objective The goal of this study was to evaluate the remediation potency of lactobacilli against chromate and the protective effects of lactobacilli against chromium-induced toxicity in mice. We also evaluated the mechanism of a new approach – “gut remediation” – by testing whether probiotics directly remediate heavy metals or indirectly by modifying the microbial community.

Design Fifty female Kunming mice were randomly divided into five experimental groups. Lactobacillus plantarum TW1-1 was orally administrated every other day for 7 weeks during exposure to 0.1 mM potassium bichromate in drinking water. The effects of TW1-1 on chromium concentrations in feces and tissues, histopathological changes, the alterations in enzymatic activity and levels in tissues, and the Cr(VI) reduction ability of fecal microbes were examined. Combined histology, quantitative real-time PCR, and microbiota phylogenetic profiling analyses were used. Moreover, overall structural changes of gut microbiota following Cr(VI) treatment and TW1-1 intervention were analyzed via MiSeq sequencing.

Results Experiments with mice showed that oral administration of strain TW1-1 might help to protect against chromium by decreasing chromium levels in tissues and increasing chromium excretion in feces, and may also attenuate alterations in oxidative stress and inflammation, and histopathological changes caused by chromium exposure. Moreover, strain TW1-1 modulated gut microbiota to alleviate chromium toxicity. The most significant finding of this study was that the probiotic remediates heavy metal not only directly but also indirectly by altering the gut microbial community. We call this process of probiotic remediation of heavy metal in the gut “gut remediation.”

Conclusion This study demonstrated the utility of lactobacillus TW1-1 in attenuating Cr(VI) toxicity. TW1-1 removes Cr from the host and acts as a regulator of gut microbiota, which protected the host from Cr toxicity. Our results indicate that gut remediation has potential for mitigating heavy metal pollution of foods.