发布时间 :2012-02-14  阅读次数 :1938

报告题目: Actinobacteria, a treasure box of P450s: How we can use them in  Biocatalysis?
报告人:Prof. Byung-Gee Kim
·Professor in School of Chemical and Biological Engineering, Seoul National University ;
·Ph.D. degree in in Food Science and Technology, Cornell University in 1989;
·Research Interest:(1) Biocatalysis and Enzyme Reaction Engineering
(2)Proteomics and Application of Mass spectrometry
报告时间: 2012年02月17(周五)下午01:30
报告地点: 生命科学技术学院3号楼105室
Actinobacteria are treasure box of P450s, dioxygenases and tyrosinases which can perform various oxidation reactions. Using the regiospecific hydroxylation of (iso)flavonoids( e.g. daidzein, genistein, apigenin etc.) as a model system, we have identified that both tyrosinase and P450s are greatly involved in the hydroxylation. Removal of tyrosinase activity is critical to the reduction in the degradation of (iso)flavonoids and maintain the regiospecific hydroxylation exerted by P450s. It will be discussed that how we can improve various reaction parameters of P450, such as oxygen and substrate transfer rate, electron transfer efficiency, and reaction rate constants, hence reaction productivity and yields by using protein engineering as well as host cell development. In addition, it will be discussed that how we can reduce the degradation of (iso)flavonoids using various strategies, such as deletion of dioxygenase and/or tyrosinase, metal ion limitation, introduction of reversible inhibitors, etc.