Biotechnology Paradigm Shift: From Living Microorganisms to Cell-Free Biosystems
【报告人】：Y.-H. Percival Zhang (张以恒), Ph.D.
Biological Systems Engineering Department, Virginia Tech, 304 Seitz Hall, Blacksburg, Virginia 24061, USA
Cell-free biosystems for manufacturing (CFBM) are the implementation of complicated biological reactions via the in vitro assembly of numerous (stable) enzymes originated from different sources and/or (biomimetic) cofactors 1-5. CFBM features numerous industrial production advantages: high product yield, fast reaction rate, easy access and control for open systems, tolerance of toxic compounds, broad reaction conditions, and so on. In this talk, I will present numerous CFBM examples, for example, the theoretical yield hydrogen production from sugars (e.g., pentoses and hexoses) 6-8, enzymatic conversion of cellulose to starch (amylose) 9, and (iii) super-energy density sugar-powered biobatteries. CFBM would become a high-yield and low-cost manufacturing platform and lead to a biotechnology paradigm shift, especially in the sustainability revolution pertaining to the energy-water-food nexus 10,11.
1 Zhang, Y.-H. P., Sun, J.-B. & Zhong, J.-J. Biofuel production by in vitro synthetic pathway transformation. Curr. Opin. Biotechnol. 21, 663-669 (2010).
2 Rollin, J. A., Tam, W. & Zhang, Y. H. P. New biotechnology paradigm: cell-free biosystems for biomanufacturing. Green Chem., DOI: 10.1039/C1033GC40625C, doi:10.1039/C3GC40625C (2013).
3 Zhang, Y.-H. P., Myung, S., You, C., Zhu, Z. G. & Rollin, J. Toward low-cost biomanufacturing through cell-free synthetic biology: bottom-up design. J. Mater. Chem. 21, 18877-18886 (2011).
4 Zhang, Y.-H. P. Production of biocommodities and bioelectricity by cell-free synthetic enzymatic pathway biotransformations: Challenges and opportunities. Biotechnol. Bioeng. 105, 663-677 (2010).
5 Zhang, Y.-H. P. Simpler is better: high-yield and potential low-cost biofuels production through cell-free synthetic pathway biotransformation (SyPaB). ACS Catal. 1, 998-1009 (2011).
6 Martín del Campo, J. S. et al. High-Yield Production of Dihydrogen from Xylose by Using a Synthetic Enzyme Cascade in a Cell-Free System. Angew. Chem. Int. Ed. 52, 4587-4590, doi:10.1002/anie.201300766 (2013).
7 Zhang, Y.-H. P., Evans, B. R., Mielenz, J. R., Hopkins, R. C. & Adams, M. W. W. High-yield hydrogen production from starch and water by a synthetic enzymatic pathway. PLoS One 2, e456 (2007).
8 Ye, X. et al. Spontaneous high-yield production of hydrogen from cellulosic materials and water catalyzed by enzyme cocktails. ChemSusChem 2, 149-152, doi:10.1002/cssc.200900017 (2009).
9 You, C. et al. Enzymatic transformation of nonfood biomass to starch. Proc. Nat. Acad. Sci. USA 110, 7182-7187, doi:10.1073/pnas.1302420110 (2013).
10 Zhang, Y.-H. P. Next generation biorefineries will solve the food, biofuels, and environmental trilemma in the energy-food-water nexus. Energy Sci. Eng. 1, 27-41 (2013).
11 Zhang, Y.-H. P. & Huang, W.-D. Constructing the electricity-carbohydrate-hydrogen cycle for a sustainability revolution. Trends Biotechnol. 30, 301-306 (2012).