报告题目：Innovative production of fuels and chemicals from renewable resources
报 告 人：Dr. Jinchuan Wu
联 系 人：秦浩/34206350
A 2-step process for biodiesel production was developed, which allows the use of crude vegetable oils containing fatty acids or water and produces salt-free glycerol directly. A method for converting “hard” substrates such as xylose, arabinose and glycerol to biogas was developed by adding exogenous microbes that are able to convert these “hard” substrates into biogas intermediates. A method for genome shuffling to improve microbes was developed and successfully utilized to improve the ethanol tolerance of a yeast strain and acid tolerance of a lactic acid bacterium. A method for introducing random point mutations to small size DNAs was developed and used for directed evolution of the signal peptide of a glucoamylase. Oil palm empty fruit bunch (EFB) was hydrolyzed to get hemicellulose sugars by the combined use of dilute H2SO4 and H3PO, giving sugars at >100 g/L in the hydrolysate without any additional concentration steps. Furfural, 5-hydroxymethyl furfural and acetic acid in hydrolysate were efficiently degraded by simply adding the microorganisms that were isolated from the nature. Cellulose was hydrolyzed to glucose by using whole cell cultures without supplementing any exogenous enzymes. Thermophilic bacteria were isolated from the natural environment and used to convert all lignocellulose sugars to L-lactic acid at 50°C in a simple medium with high yield (>95%), titer (>200 g/L) and productivity (>5 g/L/h).
Dr. Jinchuan Wu （吴金川） is a Senior Scientist and Head of the Industrial Biotechnology Division at the Institute of Chemical & Engineering Sciences (ICES), A*STAR, Singapore. He got his Bachelor, Master and PhD from Tianjin University (China) and had once worked in Kanazawa University (Japan) as an Associate Professor on 1998-2000 and in Tianjin University as a full Professor before he joined ICES in 2002. His current research of interest includes biomass conversion to fuels and chemicals, microbial screening and fermentation, protein engineering, metabolic engineering and bioprocess engineering. He has over 100 publications in peer-viewed international journals and 14 patents/invention disclosures.