报告题目：Building synthetic metabolic pathways in Saccharomyces cerevisiae for commercial production of farnesene, an important renewable chemical

报告人：Lishan Zhao 博士

美国Amyris公司研发主任

报告时间： 12月5日 9:30

报告地点：闵行校区生物药学楼3-105

联系人：秦浩（钟建江教授课题组）34206350

Abstract：β-Farnesene is a commercially valuable molecule whose derivatives can be used in a wide variety of applications ranging from fuels to novel performance chemicals. At Amyris, we have used the native glycolytic pathway of Saccharomyces cerevisiae and a heterologous farnesene synthase to produce farnesene at industrial scale from plant-derived sugars.  Generating farnesene using the native pathway, however, results in an imbalance of pathway metabolites and cofactors, translating to high energetic costs to the cell and overall higher production costs. Here, we design and construct an alternative metabolic pathway in S. cerevisiae which reduces the cost of converting glucose to farnesyl pyrophosphate (FPP), the precursor to farnesene and all other sesquiterpenes. By dividing glucose dissimilation between parallel heterologous routes, and by altering the cofactor requirements of FPP biosynthesis, we dramatically improve internal pathway balance, reducing the demand to maintain redox homeostasis and energy charge using side reactions. In this presentation, I will highlight how we rewired the yeast metabolic network and created an engineered strain with higher theoretical yield and improved productivity, enabling microbial production of farnesene at commercial scale with significantly reduced cost.