发布时间 :2015-07-05  阅读次数 :2348

报告题目:A novel mechanism of small RNA biogenesis in a causative protozoan- a data driven model generation

报  告 人: 张力烨 博士

Boston University School of Medicine

Department of Medicine, Computational Biomedicine



联  系 人:张晓君 This e-mail address is being protected from spambots. You need JavaScript enabled to view it.


Abstracts:Small RNA biogenesis (such as microRNA and siRNA) typically involves cleavage of structured precursors by RNase III-like endonucleases. However, guide RNAs that direct U-insertion/deletion mRNA editing in mitochondria of trypanosomes maintain 5′ triphosphate characteristic of transcription start site and possess U-tail indicative of 3′ processing and uridylation. Here, we will show you how computational analysis integrates multiple RNA-Seq assays (Stranded RNA-Seq, CLIP-Seq and RNA-IP-Seq) and guides biochemistry experiments and then lead to a completely novel model for guide RNA generation in which gRNA is derived from the uridylation-induced, antisense transcription-controlled exonucleolytic degradation.