Developingproteincomplexpurificationandgenomeengineeringtoolsforstudyingplantdiseaseresistance

发布时间 :2013-06-18  阅读次数 :653

 

【报告题目】: Developing protein complex purification and genome engineering tools for studying plant disease resistance

【报 告 人】: Dr. Yiping Qi

Center for Microbial and Plant Genomics

University of Minnesota-twin cities

1500 Gortner Ave

St. Paul, MN, 55108, USA

Email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it.

【报告时间】: 2013年6月18日(星期二)下午14:00时

【报告地点】: 上海交通大学生命学院1-105号会议室

【联系人】:  张大兵 教授

 

报告人背景介绍:

EDUCATION

9/2003-10/2009 University of Minnesota, Twin Cities, MN

Ph.D., Plant Biological Sciences

9/2000-3/2003 Shanghai Jiao Tong University, Shanghai, China

M.S., Biochemistry and Molecular Biology

9/1996-6/2000 Nankai University, Tianjin, China

B.S., Microbiology

PUBLICATIONS

 

Postdoc research publications:

Yiping Qi, Yong Zhang, Joshua A. Baller and Daniel F. Voytas. Histone H2AX and small RNAs modulate both NHEJ and HR DNA repair in plants. (In preparation)

Michelle L. Christian, Yiping Qi, Yong Zhang and Daniel F. Voytas. Targeted mutagenesis in Arabidopsis thaliana using Engineered TAL effector nucleases (TALENs). (Submitted to Genetics)

Yiping Qi, Xiaohong Li, Yong Zhang, Feng Zhang, Colby Starker, Nicholas J. Baltes, Jeffry D. Sander, Deepak Reyon, Drena Dobbs, J Keith Joung, Daniel F. Voytas. Targeted deletion and inversion of tandemly arrayed genes in Arabidopsis thaliana using zinc finger nucleases. (Under revision for G3)

 

Yiping Qi, Colby Starker, Feng Zhang, Nicholas Baltes and Daniel Voytas. Tailor-made mutations in Arabidopsis using zinc finger nucleases. In: Methods in Molecular Biology, Humana Press, (In press)

Yiping Qi, Yong Zhang, Feng Zhang, Joshua A. Baller, Spencer C. Cleland, Yungil Ryu, Colby G. Starker and Daniel F. Voytas. Increasing frequencies of site-specific mutagenesis and gene targeting in Arabidopsis by manipulating DNA repair pathways. Genome Research, 2013 Jan 2. [Epub ahead of print]

Yong Zhang, Feng Zhang, Xiaohong Li, Joshua Baller, Yiping Qi, Colby G. Starker, and Daniel F. Voytas. High efficiency plant genome engineering using TALENs. Plant Physiology, 2013, 161(1):20-7

Jeffry D Sander,Elizabeth J Dahlborg,Mathew J Goodwin,Lindsay Cade,Feng Zhang,Daniel Cifuentes,Shaun J Curtin,Jessica S Blackburn,Stacey Thibodeau-Beganny,Yiping Qi,Christopher J Pierick,Ellen Hoffman,Morgan L Maeder,Cyd Khayter,Deepak Reyon,Drena Dobbs,David M Langenau,Robert M Stupar,Antonio J Giraldez,Daniel F Voytas,Randall T Peterson,Jing-Ruey J Yeh& J Keith Joung. Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA). Nature Methods, 2011,8: 67-69

PhD research publications:

Hua Shi, Qiujing Shen, Yiping Qi, Haozhen Nie, Yongfang Chen, Ting Zhao,Fumiaki Katagiri, Dingzhong Tang. Arabidopsis BR-SIGNALING KINASE1 physically associates with the PAMP receptor FLAGELLIN SENSING2 and regulates plant innate immunity. (Plant Cell, in press)

Kenichi Tsuda, Yiping Qi, Le V. Nguyen, Gerit Bethke, Yayoi Tsuda, Jane Glazebrook, and Fumiaki Katagiri. An efficient Agrobacterium-mediated transient transformation of Arabidopsis. The Plant Journal, 2012, 69 (4): 713-719

Yiping Qi and Fumiaki Katagiri. A membrane microdomain may be a platform for immune signaling. Plant Signaling & Behavior, 2012, 7 (4): 454-456

Yiping Qi, Kenichi Tsuda, Le V. Nguyen, Xia Wang, Jinshan Lin, Angus S. Murphy, Jane Glazebrook, Hans Thordal-Christensen and Fumiaki Katagiri. Physical association of Arabidopsis hypersensitive induced reaction proteins (HIRs) with the immune receptor RPS2. The Journal of Biological Chemistry, 2011, 286 (36): 31297-31307

Yiping Qi, Kenichi Tsuda, Jane Glazebrook and Fumiaki Katagiri. Physical association of PTI and ETI immune receptors in Arabidopsis. Molecular Plant Pathology,2011, 12 (7): 702-708

Yiping Qi, Fumiaki Katagiri. Purification of resistance protein complexes using a biotinylated affinity tag. In: Methods in Molecular Biology, Humana Press, 2011, 712: 21-30

Yiping Qi, Kenichi Tsuda, Anna Joe, Masanao Sato, Le V. Nguyen, Jane Glazebrook , James R. Alfano, Jerry D. Cohen and Fumiaki Katagiri. A putative RNA-binding protein positively regulates salicylic acid-mediated immunity in Arabidopsis. Molecular Plant Microbe Interaction, 2010, 23(12): 1573-1583

Yiping Qi, Fumiaki Katagiri. Purification of low-abundance Arabidopsis plasma- membrane protein complexes and identification of candidate components. The Plant Journal, 2009, 57(5): 932-944

 

Master research publications:

Yiping Qi, Yue Sun, Lin Xu, Yuquan Xu, Hai Huang. ERECTA is required for protection against heat-stress in the AS1/AS2 pathway to regulate adaxial-abaxial leaf polarity in Arabidopsis. Planta. 2004, 219: 270-276

Yiping Qi, Yijian He, Yuquan Xu. Rhizobacteria-mediated Induced Systemic Resistance in plant. Plant Physiology Communications. 2003, 39(3): 273-278

Yijian He,Yiping Qi, Yuquan Xu. An improved method for inducing systemic resistance in Arabidopsis thaliana. Journal of Plant Physiology and Molecular Biology. 2003, 29(1): 75-798.

 

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