Litao Yang

Education and Research Experience

  • Sep, 1998 to June, 2002 Nanjing University, China. B.S.     
  • Sep, 2002 to June, 2006 Nanjing University, China.  Ph.D.   
  • July, 2006 to Dec, 2009   Assistant Prof, School of Life Science and Biotechnology, Shanghai JiaoTong University, China.
  • June, 2008 to July, 2008  Visiting scholar, Institute for Agricultural and Fisheries Research (ILVO), Belgium.
  • July, 2008 to Sep, 2008  Visiting scholar, National Institute of Biology, Slovenia.
  • Jan, 2010 to Dec, 2016  Associate Professor, School of Life Science and Biotechnology, Shanghai JiaoTong University, China.
  • Since Jan, 2017             Professor, School of Life Science and Biotechnology, Shanghai JiaoTong University, China.

Selected Publications

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    Zaobing Zhu, Rong Li, Hanwen Zhang, Jinyue Wang, Yongyi Lu, DabingZhang, LitaoYang. PAM-free loop-mediated isothermal amplification coupled with CRISPR/Cas12a cleavage (Cas-PfLAMP) for rapid detection of rice pathogens. Biosensors and Bioelectronics. 2022 Feb12 . doi: 10.1016/j.bios.2022.114076


    Zhang H, Li R, Guo Y, Zhang Y, Zhang D, Yang L. LIFE-Seq: a universal Large Integrated DNA Fragment Enrichment Sequencing strategy for deciphering the transgene integration of genetically modified organisms. Plant Biotechnol J. 2022 Jan 6. doi: 10.1111/pbi.13776.


    Liu Q, Guo X, Xun G, Li Z, Chong Y, Yang L, Wang H, Zhang F, Luo S, Cui L, Zhao P, Ye X, Xu H, Lu H, Li X, Deng Z, Li K, Feng Y. Argonaute integrated single-tube PCR system enables supersensitive detection of rare mutations. Nucleic Acids Res. 2021 Apr 27:gkab274. doi: 10.1093/nar/gkab274.


    Yijie Wang, Rong Li, Zaobing Zhu, Zheng Yuan, Chen Wang, Li Wang, Dabing Zhang, Litao Yang. In vitro Argonaute cleavage-mediated quantitative PCR facilitates versatile CRISPR/Cas-induced mutant analysis. Sensors and Actuators B: Chemical, 2023, 374, 132781.
    Xueqi Li, Rong Li, Zheng Yuan, Zaobing Zhu, Wenting Xu, Yijie Wang, Dabing Zhang, and Litao Yang*. One Versatile Cas9-Integrated Single-Tube Duplex Quantitative Real-Time PCR System for Rapid Analysis of CRISPR/Cas-Induced Mutants. Anal. Chem. 2022, 94, 30, 10832–10840.
    Xu W, Zhang H, Zhang Y, Shen P, Li X, Li R, Yang L. A paired-end whole-genome sequencing approach enables comprehensive characterization of transgene integration in rice. Commun Biol. 2022 Jul 5;5(1):667.

    • Zaobing Zhu, Yongkun Guo, Chen Wang, Zifeng Yang, Rong Li, Zhiqi Zeng, Hui Li, Dabing Zhang, Litao Yang. An ultra-sensitive one-pot RNA-templated DNA ligation rolling circle amplification-assisted CRISPR/Cas12a detector assay for rapid detection of SARS-CoV-2. Biosensors and Bioelectronics, 2023, 115179.
    • Yanan Meng, Shu Wang, Jinchao Guo, Litao Yang. Collaborative Ring Trial of the Applicability of a Reference Plasmid DNA Calibrant in the Quantitative Analysis of GM Maize Event MON810. Foods 2022, 11(11), 1538;
    • Wenting Xu,Ping Shen,Rong Li,Biao Liu andLitao Yang. Development of an Event-Specific Droplet Digital PCR Assay for Quantification and Evaluation of the Transgene DNAs in Trace Samples of GM PRNP-Knockout Goat. Foods 2022, 11(6), 868
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    Hanwen Zhang, Yuchen Zhang, Wenting Xu, Rong Li, Dabing Zhang, Litao Yang*. Development and performance evaluation of whole-genome sequencing with paired-end and mate-pair strategies in molecular characterization of GM crops: One GM rice 114-7-2 line as an example. Food Chemistry: Molecular Sciences, 2022;4 :100061

    Rong Li, Wenting Xu, Xiujie Zhang, Xueqi Li, Jinjie Cui, and Litao Yang*Ultrasensitive Hexaplex Droplet Digital Polymerase Chain Reaction Assay for Rapid Screening and Quantification of Genetically Modified Content. ACS Agric. Sci. Technol. 2021;1, 390−399 DOI: 10.1021/acsagscitech.1c00104

    • Yang L, Chen Y, Li R, Xu W, Cui J, Zhang D, Zhang X. Universal LNA Probe-Mediated Multiplex Droplet Digital Polymerase Chain Reaction for Ultrasensitive and Accurate Quantitative Analysis of Genetically Modified Organisms. J Agric Food Chem. 2021 Feb 10;69(5):1705-1713
    • Li R, Chen J, Zhang X, Cui J, Tao S, Yang L. Mini-Disk Capillary Array Coupling with LAMP for Visual Detection of Multiple Nucleic Acids using Genetically Modified Organism Analysis as an Example. J Agric Food Chem. 2020 Jan 22;68(3):899-906.
    • Zhang Y, Zhang H, Qu Z, Zhang X, Cui J, Wang C, Yang L. Comprehensive analysis of the molecular characterization of GM rice G6H1 using a paired-end sequencing approach.  Food Chem. 2020 Mar 30;309:1257-60.
    • Rong Li, Yan Ba, Yu Song, Litao Yang. Rapid and sensitive screening and identification of CRISPR/Cas9 edited rice plants using quantitative real-time PCR coupled with high resolution melting analysis. Food Control. 2020, 112, 107088
    • Li J, Chen Y, Zheng T, Kong L, Zhu S, Sun Y, Deng Z, Yang L*, You D*. Quantitative mapping of DNA phosphorothioatome reveals phosphorothioate heterogeneity of low modification frequency. PLoS Genet. 2019; 15(4):e1008026.
    • Yang Y, Li L, Yang H, Li X, Zhang X, Xu J, Zhang D, Jin W, Yang L*. Development of Certified Matrix-Based Reference Material as a Calibrator for Genetically Modified Rice G6H1 Analysis. J Agric Food Chem. 2018; 66:3708-3715.
    • Shao N, Chen J, Hu J, Li R, Zhang D, Guo S, Hui J, Liu P, Yang L*, Tao SC*. Visual detection of multiple genetically modified organisms in a capillary array. Lab on A Chip. 2017; 17(3):521-529
    • Cheng F, Wu J, Zhang J, Pan A, Quan S, Zhang D, Kim H, Li X, Zhou S, Yang L*. Development and inter-laboratory transfer of a decaplex polymerase chain reaction assay combined with capillary electrophoresis for the simultaneous detection of ten food allergens. Food Chem. 2016. 199:799-808.
    • Pi L, Li X, Cao Y, Wang C, Pan L, Yang L*. Development and application of a multi-targeting reference plasmid as calibrator for analysis of five genetically modified soybean events. Anal Bioanal Chem. 2015, 407: 2877-86.
    • Shao N, Jiang SM, Zhang M, Wang J, Guo SJ, Li Y, Jiang HW, Liu CX, Zhang DB, Yang L*, Tao SC. MACRO: A Combined Microchip-PCR and Microarray System for High-Throughput Monitoring of Genetically Modified Organisms. Anal Chem. 2014. 86: 1269-1276.
    • Zhang M, Liu Y, Chen L, Quan S, Jiang S, Zhang D, Yang L. One simple DNA extraction device and its combination with modified visual loop-mediated isothermal amplification for rapid on-field detection of genetically modified organisms. Anal Chem. 2013. 85: 75-82.