Develop genome engineering tools and smart response systems in mushroom for applicatons in efficient biosynthesis of secondary metabolites.
Develop and apply genome engineering tools to discover the genetic determinants for chassis cell construction regarding efficient biomanufacturing.
研究方向
Mushroom Synthetic Biology
Develop genome engineering tools and smart response systems in mushroom for applicatons in efficient biosynthesis of secondary metabolites.
Efficient Biomanufacturing
Develop and apply genome engineering tools to discover the genetic determinants for chassis cell construction regarding efficient biomanufacturing.
代表论著
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Qin Wang, Ye Li, Shunhan Zhang, Wei Yuan, Zeqian Du, Ting Shi, Zhao Chang, Xingye Zhai, Yinhua Lu, Meng Wang, Juan Guo, Jian-Jiang Zhong and Han Xiao* (2025). Decoding and reprogramming of the biosynthetic networks of mushroom derived bioactive type II ganoderic acids in yeast. Cell Discov, 11(1): 61.
Qin Wang, Zeqian Du, Zhenhao Li, Wei Yuan, Jian-Jiang Zhong, Juan Guo, Shenggan Luo, Ting Shi* and Han Xiao* (2025). A branchpoint cytochrome P450 CYP512A13 interconverts different types of Ganoderma triterpenoids. ACS Catal, 15, 13428–13443.
Yuhuan Luo, Jian-Jiang Zhong* and Han Xiao* (2025). Mechanism and engineering of endoplasmic reticulum-localized membrane protein folding in Saccharomyces cerevisiae. Metab Eng, 90: 43-56.
Yuhuan Luo, Zeqian Du, Chenjian Jiang, Ziqian Yu, Jian-Jiang Zhong*, Ting Shi* and Han Xiao* (2023). Rational engineering of a membrane-anchored promiscuous cytochrome P450 for efficient biosynthesis of valuable ganoderic acids. ACS Catal, 13: 15673-15681.
Wei Yuan, Chenjian Jiang, Qin Wang, Yubo Fang, Jin Wang, Meng Wang* and Han Xiao* (2022). Biosynthesis of mushroom-derived type II ganoderic acids by engineered yeast. Nat Commun, 13: 7740.
Yubo Fang and Han Xiao* (2022). The aspartic protease Yps3p and cell wall glucanase Scw10p are novel determinants that enhance the secretion of the anti-tumor triterpenoid GA-HLDOA in Saccharomyces cerevisiae. ACS Synth Biol, 11(9):2917-2926.
Han Xiao*, Yue Zhang and Meng Wang* (2019). Discovery and engineering of cytochrome P450s for terpenoid biosynthesis. Trends Biotechnol, 37(6): 618-631.
Wen-Fang Wang, Han Xiao* (co-first author) and Jian-Jiang Zhong* (2018). Biosynthesis of a ganoderic acid in Saccharomyces cerevisiae by expressing a cytochrome P450 gene from Ganoderma lucidum. Biotechnol Bioeng, 115(7): 1842-1854.
Zehua Bao, Mohammad HamediRad, Pu Xue, Han Xiao, Ipek Tasan, Ran Chao, Jing Liang and Huimin Zhao* (2018). Genome-scale engineering of Saccharomyces cerevisiae with single nucleotide precision. Nature Biotechnol, 36(6): 505-508.