Shaobo Dai

  • Tel: +86-021
  • Email: sdai@sjtu.edu.cn
  • Address: Room C402, Shanghai Center for Systems Biomedicine, Minhang campus, Shanghai Jiaotong University, 800 Dongchuan Road, Minhang District, Shanghai
  • Lab Web: https://www.x-mol.com/groups/Dai_Shaobo
  • PI, PhD supervisor. Our lab aims to understand the functions and catalytic mechanism of key metabolic enzymes, including thiamine diphosphate dependent enzyme and protein methyltransferase, with the use of a combination of structural biology, enzyme kinetics, spectroscopy and computational chemistry. Published papers include Nature, Natur

Selected Publications

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    Zhiyong Liu, Chengliang Xiao, Shuangjun Lin, Kai Tittmann*, Shaobo Dai*. (2023) Multifaceted Role of the Substrate Phosphate Group in Transketolase Catalysis. ACS Catalysis.

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    Dai, S., Funk, L.-M., von Pappenheim, F. R., Sautner, V., Paulikat, M., Schröder, B., Uranga, J., Mata, R. A. & Tittmann, K. (2019) Low-barrier hydrogen bonds in enzyme cooperativity. Nature, 573(7775) 609-613. Recommended in F1000Prime.

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    Dai S., Horton J.R., Woodcock C.B., Wilkinson A.W., Zhang X., Gozani O., Cheng X. (2019) Structural basis for the target specificity of actin histidine methyltransferase SETD3. Nature Communications 10(1) 3541

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    Wilkinson A.W.*, Diep J.*, Dai S.*, Liu S, Ooi YS, Song D, Li TM, Horton JR, Zhang X, Liu C, Trivedi DV, Ruppel KM, Vilches-Moure JG, Casey KM, Mak J, Cowan T, Elias JE, Nagamine CM, Spudich JA, Cheng X, Carette JE, Gozani O (2019) SETD3 is an actin histidine methyltransferase that prevents primary dystocia. (*co-first authors) Nature, 565(7739) 372-376. Recommended in F1000Prime.

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    Dai S, Holt MV, Horton JR, Woodcock CB, Patel A, Zhang X, Young NL, Wilkinson AW, Cheng X. Characterization of SETD3 methyltransferase mediated protein methionine methylation. Journal of Biological Chemistry. 2020, 295, 10901-10910 (cover story).

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    Dai S, Horton JR, Wilkinson AW, Gozani O, Zhang X, Cheng X. An engineered variant of SETD3 methyltransferase alters target specificity from histidine to lysine methylation. Journal of Biological Chemistry. 2020, 295 (9), 2582-2589.

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    Affaticati P.*, Dai S.*, Payongsri P., Tittmann K., Dalby P.-A. Structural Analysis of an Evolved Transketolase Reveals Divergent Binding Modes. (2016) Scientific Reports 6 35716 (*co-first authors).

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    Dai S., Liao J., Tian J., Li H., Gao W.Y. A chemo-enzymatic cascade for the one-pot syntheses of 1-deoxy-D-xylulose 5-phosphate and 1-deoxy-D-xylulose. (2012)  Tetrahedron Letters 53 4809-4812

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    Li H., Dai S., Gao W.Y. Preparation of isotope labeled/unlabeled key intermediates in 2-methyl-D-erythritol 4-phosphate terpenoid biosynthetic pathway. (2012) Helvetica Chimica Acta 95 683-715

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    Zhihong Xiao, Jinyin Zha, Xu Yang, Tingting Huang, Shuxin Huang, Qi Liu, Xiaozheng Wang,
    Jie Zhong, Jianting Zheng, Rubing Liang, Zixin Deng, Jian Zhang*, Shuangjun Lin*, Shaobo Dai*. (2024)
    A three-level regulatory mechanism of the aldo-keto reductase subfamily AKR12D. Nature Communications, 15(2128): 1-15.