Ovule is the precursor of seed. The number and development of ovule determine the quantity and quality of seed. The development and pattern formation of embryo determine the growth and development after germination. Our group focuses on the regulatory mechanism of the initiation of ovule primordia, the differentiation and development of female gametophyte, and the development and pattern formation of embryo.
Tonoplast proton pumps establish the proton gradient that powers molecular traffic across tonoplast thereby facilitating turgor regulation and nutrient homeostasis. They also affect vacuole morphology and distribution. But how the vacuole and the tonoplast proton pumps influence plant growth and development remains unclear. Our group investigate how the vacuole morphology and distribution and tonoplast proton pumps regulate the d differentiation of germ cells and related reproductive development.
Brassinosteroid (BR) plays crucial roles in modulating plant architecture and seed yield. But BR cannot be applied in agriculture production directly because BR regulated multiple processes and could not be transcriptional regulated in different tissues. The rice mutant with reduced BR signal are dwarf with erect leaves (the ideal architecture for dense planting), but also leads to decreased reproductivity and grain yield. Enhanced BR signaling contributes to increased grain yield, but the increased plant height and leaf inclination induces lodging and reduces planting density. Our group development two solutions, one is identifying new regulators which can mediate parts of BR-regulated rice growth, the other is using tissue specific promoters to derive modified coding sequences to modulate BR signaling directly.
Two Tonoplast Proton Pumps Function in Arabidopsis Embryo Development. Jiang YT, Tang RJ, Zhang YJ, Xue HW, Ferjani A, Luan S* and Lin WH*. (2019). New Phytologist. doi: 10.1111/nph.16231.
Effective Modulating Brassinosteroids Signal to Study Their Specific Regulation of Reproductive Development and Enhance Yield. Zu SH, Jiang YT, Hu LQ, Zhang YJ, Chang JH, Xue HW, and Lin WH*. (2019). Frontiers in Plant Science. 19 (10): 980.
OsGATA7 modulates brassinosteroids-mediated growth regulation and influences architecture and grain shape. Zhang YJ, Zhang Y, Zhang LL, Huang HY, Yang BJ, Luan S, Xue HW, and Lin WH*. (2018). Plant Biotechnology Journal. doi: 10.1111/pbi.12887.
Yang BJ, Lin WH (co-first author), Fu FF, Xu ZH, and Xue HW. (2017). Receptor-like protein ELT1 Regulates Rice Growth by Promoting Brassinosteroid Signaling through Interacting with BRI1. Cell Research. doi: 10.1038/cr.2017.692.
Wu P, Gao HB, Zhang LL, Xue HW and Lin WH*. Phosphatidic acid regulates AtBZR1 activity and brassinosteroid signal. Molecular Plant. 7(2): 445-447.
Jiang WB, Huang HY, Hu YW, Zhu SW, Wang ZY, and Lin WH*. 2013. Brassinosteroid regulates seed size and shape in Arabidopsis. Plant Physiology. 162(4): 1965–77.
Huang HY, Jiang WB, Hu YW, Wu P, Zhu JY, Liang WQ, Wang ZY and Lin WH*. 2013. BR signal influences Arabidopsis ovule and seed number through regulating related genes expression by transcription factor BZR1. Molecular Plant. 6(2):456-69.
Luo XM, Lin WH (co-first author), Zhu SW, Zhu JY, Sun Y, Fan XY, Cheng ML, Hao YQ, Oh E, Tian MM, Liu LJ, Zhang M, Xie Q, Chong K, Wang ZY. 2010. Integration of light and brassinosteroid signaling pathways by a GATA transcription factor in Arabidopsis. Developmental Cell. 19, 872–883.