汪志军

副研究员

  • 电话:+86-02162933765-2061
  • 邮箱:wangzhijun@sjtu.edu.cn
  • 地址:上海市华山路1954号哲生馆206室
  • 博士,微生物代谢国家重点实验室,主要研究方向:次生代谢合成机器的结构和机理研究。

学术经历

  • 2007年起任职于上海交通大学微生物代谢国家重点实验室。2011-2013,加州大学伯克利分校,唐氏杰出学者。

研究方向

天然产物合成机器装配的候选研究主题

1.常用的抗生素如红霉素、青霉素的天然产物的合成机器。

2.洛伐他丁类的合成机器:

洛伐他丁也是天然产物,是新型降血脂药物。用于治疗高胆固醇血症和混合型高脂血症,对动脉粥样硬化和冠心病有防治作用。由洛伐他丁衍生的他丁类药物,同时也是当前癌症治疗药物研究的焦点之一。

冷冻电镜技术解析蛋白结构

1.蛋白分子机器的表达和纯化
2.液态乙烷中蛋白质冷冻样品的制备
3.冷冻电镜操作与观察
4.计算解析蛋白三维结构模型

代表论著

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    Wang J, Liang J, Chen L, Zhang W, Kong L, Peng C, Su C, Tang Y, Deng X*, and Wang Z*.  Structural basis for the biosynthesis of Lovastatin. Nature Communications. 2021 Feb 8;12(1):867.

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    Pu T, Mei Z, Zhang W, Liang W, Zhou X, Liang J*, Deng Z*, Wang Z*. An in vitro DNA Phosphorothioate Modification Reaction. Molecular Microbiology. 2020 Feb;113(2):452-463.

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    Pu T, Liang J, Mei Z, Yang Y, Wang J, Zhang W, Liang W, Zhou X, Deng Z*, Wang Z*. Phosphorothioated DNA is shielded from oxidative damage. Applied and Environmental Microbiology. 2019 Apr 4;85(8).

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    Wu H, Liang J, Gou L, Wu Q, Liang W, Zhou X, Bruce IJ, Deng Z*, Wang Z*. Recycling of Overactivated Acyls by a Type II Thioesterase during Calcimycin Biosynthesis in Streptomyces chartreusis NRRL 3882.  Applied and Environmental Microbiology.  2018 May 31;84(12). e00587-18.

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    Wu H, Liang J, Wang J, Liang W, Gou L, Wu Q, Zhou X, Bruce IJ, Deng Z*, Wang Z*. Cezomycin Is Activated by CalC to Its Ester Form for Further Biosynthesis Steps in the Production of Calcimycin in Streptomyces chartreusis NRRL 3882. Applied and Environmental Microbiology.  2018 May 31;84(12). e00586-18.

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    Zhao Y, Li L, Zheng G, Jiang W, Deng Z, Wang Z*, Lu Y*. CRISPR/dCas9-Mediated Multiplex Gene Repression in Streptomyces. Biotechnology Journal.  2018 Sep;13(9): e1800121.

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    Xie X, Liang J, Pu T, Xu F, Yao F, Yang Y, Zhao Y, You D, Zhou X, Deng Z*, Wang Z*. Phosphorothioate DNA as an antioxidant in bacteria. Nucleic Acids Research. 2012 Oct; 40(18):9115-24.

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    Wu Q, Liang J, Lin S, Zhou X, Bai L*, Deng Z and Wang Z*. Characterization of the Pyrrole Polyether Antibiotic Calcimycin (A23187) Biosynthetic Gene Cluster from Streptomyces chartreusis NRRL 3882. Antimicrobial Agents and Chemotherapy (AAC). 2011 Mar; 55(3):974-82.

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    Liang J#, Wang Z#, He X, Li J, Zhou X,Deng Z. DNA modification by sulfur: analysis of the sequence recognition specificity surrounding the modification sites. Nucleic Acids Research. 2007 Apr; 35(9):2944-54.

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    Gou L, Wu Q, Lin S, Li X, Liang J, Zhou X, An D*, Deng Z*, Wang Z*. Mutasynthesis of Pyrrole Spiroketal Compound by Using Calcimycin 3-Hydroxy Anthranilic Acid Biosynthetic Mutant. Applied Microbiology and Biotechnology. 2013 Sep;97(18):8183-91.