Guangyu Yang

Research Professor

  • Tel: +86-021-34205079
  • Email: yanggy@sjtu.edu.cn
  • Address: School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, P. R. China, 200240
  • Lab Web: http://enzyme.sjtu.edu.cn/yjry_v.asp?upid=114&idh=7
  • Prof.Yang’s research mainly focus on enzyme engineering and synthetic biology.He has more than 40 high quality articles published in prestigious journals including Sci Adv.,Nature Commun.,Angewe Chemie, et.al.He has won the Tang Lixin Outstanding Scholar Award (2017) and Shanghai Bioengineering "Tofflon" Outstanding Young Scientist(2019).

Education and Research Experience

  • Education
  • Mar. 2012-June. 2013   Postdoc fellow, University of British Columbia (Supervisor: Prof. Stephen G. Withers)
  • Nov. 2007-Oct. 2008    Jointly educated Ph.D student, University of British Columbia (Supervisor: Prof. Stephen G. Withers)
  • Mar. 2007-June. 2007   Visiting student, Seoul National University (Supervisor: Prof. Young Je Yoo)
  • Sept. 2003-July. 2009    Ph.D in Molecular Biology and Biochemistry, Jilin University, China (Supervisor: Prof. Yan Feng)
  • Sept. 1999-July. 2003    BS in Biological Science, Jilin University, China
  • Research experiences
  • Jan. 2018-Present        Professor, Doctoral supervisor, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University
  • Jan. 2013-Dec. 2017    Associate professor, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University
  • July. 2009-Dec. 2012   Lecturer, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University

Selected Publications

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    Yumeng Tan, Yong Zhang, Yunbin Han, Hao Liu, Haifeng Chen, Fuqiang Ma, Stephen G Withers, Yan Feng, Guangyu Yang. Directed evolution of an α1,3-fucosyltransferase using a single-cell ultrahigh-throughput screening method. Science Advances, 2019,5(10): eaaw8451.

    PDF SUPP INOF
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    Fuqiang Ma#, Meng Ting Chung#, Yuan Yao, Robert Nidet, Lap Man Lee, Allen P. Liu, Yan Feng, Katsuo Kurabayashi*, Guang-Yu Yang*. Efficient molecular evolution to generate enantioselective enzymes using a dual-channel microfluidic droplet screening platform. Nature Communications, 2018, 9:1030. (#equal contribution)

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    Zhuang Y#, Yang GY#, Chen X, Liu Q, Zhang X, Deng Z, Feng Y. Biosynthesis of plant-derived ginsenoside Rh2 in yeast via repurposing a key promiscuous microbial enzyme. Metabolic Engineering. 2017, 42:25-32. (#equal contribution)

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    Han YB, Chen LQ, Li Z, Tan YM, Feng Y, Yang GY*. Structural insights into the broad substrate specificity of a novel Endo-glycoceramidase I belonging to a new subfamily of GH5 glycosidase. J Biol Chem. 2017, 292(12):4789-4800.

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    Fuqiang Ma, Yuan Xie, Manjie Luo, Shuhao Wang, You Hu, Yukun Liu, Yan Feng, Guang-Yu Yang*. Sequence Homolog-based Molecular Engineering for Shifting the Enzymatic pH Optimum. Synthetic and Systems Biotechnology, 2016, 1, 195-206.

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    Fuqiang Ma, Michael Fischer, Yunbin Han, Stephen G. Withers, Yan Feng, Guang-Yu Yang*. Substrate engineering enabling fluorescence droplet entrapment for IVC-FACS-based ultrahigh-throughput screening. Analytical Chemisty, 2016, 88, 8587−8595.

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    Guang-Yu Yang, Caishun Li, Michael Fischer, Christopher W. Cairo, Yan Feng, Stephen G. Withers. A FRET Probe for Cell-Based Imaging of Ganglioside-Processing Enzyme Activity and High-Throughput Screening. Angewandte Chemie International Edition. 2015, 54(18):5389-5393

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    Yun-Bin Han, Lie Wu, Jamie R. Rich, Feng-Tao Huang, Stephen G. Withers, Yan Feng, Guang-Yu Yang*. Comprehensive Characterization of Sphingolipid Ceramide N-deacylase for the Synthesis and Fatty Acid Remodeling of Glycosphingolipids. Applied Microbiology and Biotechnology. 2015, 99(16):6715-6726.

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    FT Huang, YB Han, Y Feng, GY Yang*. A facile method for controlling the reaction equilibrium of sphingolipid ceramide N-deacylase for lyso-glycosphingolipid production. Journal of lipid research. 2015, 56(9):1836-1842.

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    Guangyu Yang, Jamie R. Rich, Michel Gilbert, Warren W. Wakarchuk, Yan Feng, Stephen G. Withers. Fluorescence Activated Cell Sorting as a General Ultra High-throughput Screening Method for Directed Evolution of Glycosyltransferases. J. Am. Chem. Soc., 2010，132(30):10570-10577.

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Academic Rewards

  • In 2019, he won the “Tofflon Young Scientists Award”of Shanghai Society of Biotechnology.
  • In 2017, he won the "Tang Lixin Outstanding Scholar Award". 
  • In 2017, he won the title of "Morningstar Outstanding Young Scholar" of Shanghai Jiao Tong University. 
  • In 2016, he won the SMC Morningstar Scholars Outstanding Young Teacher Award Program of Shanghai Jiao Tong University (Tier A). 
  • In 2013, he was awarded the SMC Morningstar Scholars Outstanding Young Teacher Award Program of Shanghai Jiao Tong University (Tier B). 
  • In 2010, he was awarded the SMC Morningstar Scholars Outstanding Young Teacher Award Program of Shanghai Jiao Tong University (Tier C). 

 






 

 

 

Teaching Experiences

  • 2017-2018-1  Advanced Biochemistry (BI28011); 
  • 2016-2017-1  Protein Engineering and Its Applications; 
  • 2016-2017-1  Structure and Function of Biological Macromolecules; 
  • 2016-2017-1  Bioengineering Unit Operation Experiment (2); 
  • 2014-2015-2  Biochemistry (class B) (2); 
  • 2014-2015-1  Biochemistry (class B) (1); 
  • 2013-2014-1  The Evolution and Future of Enzymes.
  • Molecular evolution of uridine phosphorylase, enterprise-funded, 2019.09.10-2020.09.09, 600k RMB, PI;
  • Design and expression of engineering enzyme based on the principle of "concave-convex complementarity" in chemical biology, National Key Research and Development Program, 2019.08-2024.07, 2,480k RMB, PI;
  • Single-cell ultra-high throughput screening method for arginine producing strains, enterprise-funded,  2019.08.01-2019.12.31, 500k RMB, PI;
  • Application of mass spectrometry to diagnose the changes of eyelid in patients with meibomian gland dysfunction and evaluation of clinical therapeutic effect, medical-industrial cross youth project of Shanghai Jiao Tong University, 2019.01.01-2021.12.31,  100k RMB, PI;
  • The development of a high-throughput microbial evolution instrument, a major instrument project of the National Natural Science Foundation of China (sub-project), 2017.01-2021.12,  948k RMB, PI;
  • Global structure-activity relationship analysis and molecular modification of sphingolipid glycoside synthase, a project supported by the National Natural Science Foundation of China, 2017.01-2020.12,  600k RMB, PI;
  • Study on the early warning role and mechanism of HPCAL1 in the malignant transformation of cholecystitis (sub-project), school project, 2017.01-2019.12, 120k RMB, participate;
  • Enzymatic conversion synthesis of ginsenoside Rh2, enterprise-funded, 2017.01.01-2017.06.31, 400k RMB, PI;
  • Development of artificial biosynthesis system of ganglioside GM1, enterprise-funded, 2016.01.01-2018.12.31, 4,500k RMB, PI;
  • Action Mechanism and Molecular Evolution of Metabolism-related enzymes, Special Fund for Scientific and technological Innovation of Shanghai Jiao Tong University, 2015.12-2016.11, 1,000k RMB, PI;
  • Development of cephalosporin C acylase, enterprise-funded,  2015.08.01-2018.07, 300k RMB, PI;
  • Molecular modification of the selectivity of non-natural donor substrates by fucosyltransferase, Project supported by the National Natural Science Foundation of China, 2015.01-2018.12, 850k RMB, PI;
  • Ultra-high throughput enzyme screening system based on "FACS-enzyme microreactor", Youth Foundation of the National Natural Science Foundation of China, 2012.01-2014.12, 230k RMB, PI;
  • In vitro multi-enzyme coupling system assembly of gangliosides, national key basic research and development plan. (project 973) sub-project, 2012.01-2013.12, 500k RMB, PI;
  • Ultra-high throughput screening method of lipase based on "FACS-enzyme microreactor" and its application in directed evolution, Fund for New Teachers of the Ministry of Education, 2012.01-2013.12, 40k RMB, PI;
  • Artificial synthetic Cell Factory, a sub-project of the National key basic Research and Development Program (973 Program), 2011.01-2016.01, 1,380k RMB, PI;
  • Ultra-high throughput single cell enzyme activity screening system based on microfluidic control and droplet sorting, enterprise-funded, 2011.08-2014.08,  300k RMB, PI;

 

Selected Grants