Xinyi He

Research Professor

Selected Publications

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    Xiankun Wang, Yuchun Zhao, Yaojie Gao, Xiangkun Luo, Aiqing Du, Zixin Deng, Zabriskie TM, Xinyi He*, Ming Jiang*. A [3Fe-4S] cluster and tRNA-dependent aminoacyltransferase BlsK in the biosynthesis of Blasticidin S. Proc Natl Acad Sci  U S A. 2021 Jul 27;118(30):e2102318118. doi: 10.1073/pnas.2102318118.

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    Hao Yu, Jiayi Li, Guang Liu, Gong Zhao, Yuli Wang, Wenyue Hu, Zixin Deng, Geng Wu, Jianhua Gan, Yi-Lei Zhao*, Xinyi He*. DNA backbone interactions impact the sequence specificity of DNA sulfur-binding domains: revelations from structural analyses.  Nucleic Acids Research 2020 Sep 4;48(15):8755-8766. doi: 10.1093/nar/gkaa574.

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    Hongliang Qian, Hao Yu, Peifei Li, E Zhu, Qingqing Yao, Cui Tai, Zixin Deng, Kenn Gerdes, Xinyi He*, Jianhua Gan*, Hong-Yu Ou*. Toxin-antitoxin operon kacAT of Klebsiella pneumoniae is regulated by conditional cooperativity via a W-shaped KacA-KacT complex. Nucleic Acids Research. 2019 Aug 22;47(14):7690-7702.

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    Guang Liu, Wencheng Fu, Zhengyi Zhang, Yao He, Hao Yu, Yuli Wang, Xiaolei Wang, Yi-Lei Zhao, Zixin Deng, Geng Wu*, Xinyi He*. Structural basis for the recognition of sulfur in phosphorothioated DNA. Nature Communications. 2018 Nov 8;9(1):4689.

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    Hao Yu, Guang Liu, Gong Zhao, Wenyue Hu, Geng Wu, Zixin Deng, Xinyi He*. Identification of a conserved DNA sulfur recognition domain by characterizing the phosphorothioate-specific endonuclease SprMcrA from Streptomyces pristinaespiralis. Molecular Microbiology. 2018 Nov;110(3):484-497.

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    Xiankun Wang, Aiqing Du, Guiyang Yu, Zixin Deng, Xinyi He*. Guanidine N-methylation by BlsL Is Dependent on Acylation of Beta-amine Arginine in the Biosynthesis of Blasticidin S. Front Microbiol. 2017 Aug 22;8:1565.

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    Gong Zhao, Geng Wu, Yan Zhang, Guang Liu, Tiesheng Han, Zixin Deng, and Xinyi He*. Structure of the N-glycosidase MilB in complex with hydroxymethyl CMP reveals its Arg23 specifically recognizes the substrate and controls its entry. Nucleic Acids Research 2014, 42(12): 8115-8124.

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    Tiesheng Han, Megumu Yamada-Mabuchi, Gong Zhao, Li Li, Guang Liu, Hong-Yu Ou, Zixin Deng, Yu Zheng*, and Xinyi He*. Recognition and cleavage of 5-methylcytosine DNA by bacterial SRA-HNH proteins. Nucleic Acids Research 2015, 43(2): 1147-1159.

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    Jun Feng, Jun Wu, Jie Gao, Zhigui Xia, Zixin Deng, and Xinyi He*. Biosynthesis of the methylarginine residue of peptidyl nucleoside arginomycin in Streptomyces arginensis NRRL 15941. Applied and Environmental Microbiology 2014, 80(16): 5021-5027.

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  • Foundations:
  • (1) Principal Investigator: Genetics and transfer mechanism of a novel genomic island present in Strepromyces lividans 66, Grant from the National Science Foundation of China (NSFC) (2006.01-2008.12). RMB300,000
  • (2) Principal Investigator: Identification and molecular design of targets for biological drugs used in agriculture, 863 grant from the Ministry of Science and Technology: (2006.01-2009.12). RMB 500,000
  • (3) Principal Investigator: Engineering of industrial erythromycin-producing strain and optimization technology of fermentation process, 863 grant from the Ministry of Science and Technology: (2006.01-2009.12). RMB 400,000
  • (4) Principal Investigator: Identification and functional characterization of a DNA sulfur modification dependent restriction system in Strepromyces coelicolor, Grant from NSFC (2010.01-2012.12). RMB350,000
  • (5) Principal Investigator: Identification and functional characterization of DNA methylation dependent restriction system in Strepromyces coelicolor, Grant from NSFC (2012.01-2015.12). RMB600,000
  • (6) Principal Investigator: Enzymatic studies on key steps and novel self-resistances involved in the biosynthesis for Blasticidin S and its analogs, Grant from NSFC (2015.01-2018.12). RMB850,000
  • (7) Principal Investigator: Genome minimization of Streptomyces FR-008 for optimal production of secondary metabolites (2011.01-2015.12). 973 grant from the Ministry of Science and Technology: RMB 800,000
  • (8) Principal Investigator: Discovery of Sulfur binding protein and functional and structural study: (2017.01-2020.12). Grant from NSFC: RMB 630,000
  • (9) Principal Investigator: Cleavage and application of DNA-dependent HNH REase (2019.01-2022.12). Grant from NSFC: RMB 630,000

Selected Grants