Xudong Kong

Tenure-track Associate Professor

  • Tel:
  • Email: xudong.kong@sjtu.edu.cn
  • Address: 2-409, Zhangheng Road 429, Postcode 201203, Pudong District, Shanghai, China
  • Our research focuses on protein and peptide evolution based on phage display and next-generation sequencing technologies.

Education and Research Experience

Education background

  • Jul. 2009 – Apr. 2015               PhD in Biochemical Engineering

                                                       Supervisors: Dr. Prof. Jian-He Xu, Dr. Prof. Jiahai Zhou

                                                       Bioengineering School

                                                       East China University of Science & Technology, China

                                                       Shanghai Institute of Organic Chemistry, China

  • Sep. 2005 – Jun. 2009             BSc in Bioengineering

                                                       Supervisors: Dr. Prof. Jian-He Xu, Dr. Jiang Pan

                                                       Bioengineering School

                                                       East China University of Science & Technology, China

Appointment

  • August 2020 – present            Tenure Track Associate Professor

                                                       School of Life Sciences and Technology

                                                       Shanghai Jiao Tong University, China

  • May 2015 – July 2020             Postdoctoral Scientist

                                                       Supervisor: Dr. Prof. Christian Heinis

                                                       Laboratory of Therapeutic Proteins and Peptides

                                                       Institute of Chemical Sciences and Engineering

                                                       EPFL, Switzerland

Research Interests

Protein fitness landscape analysis

Protein fitness landscape analysis based on phage display and next-generation sequencing techniques.

Development of orally available peptide drugs

Improve bioavailability of peptide drugs by the evolution of peptide stability and permeability.

Selected Publications

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    Han, Y., Luo, Y., Ma, B.D., Li, J.#, Xu, J.H.# and Kong, X.D.# Structural Insights of a cis-Epoxysuccinate Hydrolase Facilitate the Development of Robust Biocatalysts for the Production of l-(+)-Tartrate. Biochem., 2024, doi:10.1021/acs.biochem.4c00141

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    He, R.N., Zhang, M.J., Dai B.# and Kong, X.D.# Selection of Peptide−Bismuth Bicycles Using Phage Display. ACS Chem. Biol. 2024, doi: 10.1021/acschembio.4c00099

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    Zhu, X.X., Zheng, W.Q., Xia, Z.W., Chen, X.R., Jin, T., Ding, X.W., Chen, F.F., Chen, Q., Xu, J.H. Kong, X.D.# and Zheng, G.W.# Evolutionary insights into the stereoselectivity of imine reductases based on ancestral sequence reconstruction. Nat. Commun., 2024, 15, 10330.

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    Sun, C.*, Ma, B.D.*, Li, G., Tian, W., Yang, L., Peng, H., Lin, Z., Deng, Z., Kong, X.D.# and Qu, X.# Engineering the substrate specificity of a P450 dimerase enables the collective biosynthesis of heterodimeric tryptophan-containing diketopiperazines. Angew Chem. Int. Ed., 2023, e202304994.

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    Kong, X.D., Carle, V., Díaz-Perlas, C., Butler, K., Heinis, C.# Generation of a large peptide phage display library by self-ligation of whole-plasmid PCR product. ACS Chem. Biol., 2020, 15, 2907–2915.

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    Kale, S.S.*, Villequey, C.*, Kong, X.D.*, Zorzi, A., Deyle, K., Heinis, C#. Cyclization of peptides with two chemical bridges affords large scaffold diversities. Nat. Chem., 2018, 10, 715-723.

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    Kong, X.D., Moriya, J., Carle, V., Pojer, F., Abriata, L.A., Deyle, K., Heinis, C. De novo development of proteolytically resistant therapeutic peptides for oral administration. Nat. Biomed. Eng., 2020, 4, 560–571.

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    Kong, X.D., Yuan, S.G., Li, L., Chen, S., Xu, J.H.# and Zhou, J.# Engineering of an epoxide hydrolase for efficient bioresolution of bulky pharmaco substrates. Proc. Natl. Acad. Sci. USA, 2014, 111, 15717-15722.

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    Kong, X.D., Ma, Q., Zhou, J.#, Zeng, B.B.# and Xu, J.H.# A smart library of epoxide hydrolase variants and the top hits for synthesis of (S)-beta-blocker precursors. Angew Chem. Int. Ed., 2014, 53, 6641-6644.

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Teaching Experiences

  • Biochemistry F (fall semester)