报告题目: Generation of interneurons during postnatal and prenatal neurogenesis
报 告 人: Professor Konstantin Khodosevich,
Biotech Research and Innovation Centre (BRIC), University of Copenhagen, Denmark
联 系 人:
The majority of interneurons are generated during embryonic neurogenesis and few populations continue to be generated also postnatally. Concerted action of extrinsic stimuli is required to regulate long distance migration of newly generated neurons and their differentiation and maturation into distinct subtypes. I will present two stories from the lab where we study postnatal and prenatal neurogenesis. In one story, we identified a serotonergic mechanism that governs migration of postnatally-generated neurons in the mouse brain. Serotonergic axons originating from the raphe nuclei exhibit a conspicuous alignment with subventricular zone-derived neuroblasts. Furthermore, we showed that such alignment is evolutionary conserved from fish to mammals. Optogenetic axonal activation provides functional evidence for serotonergic modulation of neuroblast migration, and this stimulation depends on 5HT3A receptor expression in neuroblasts. In another story, we started to analyze differentiation of interneurons during prenatal neurogenesis and I will present preliminary data regarding late steps of interneuron maturation.
Konstantin Khodosevich is an associate professor at BRIC, University of Copenhagen. Two major questions that Konstantin Khodosevich’s lab addresses are: how diversity of interneurons is established during brain development and how disturbances in generation and maturation of interneurons contribute to decline of cognitive abilities in mammalian brain? His lab aims to identify intrinsic transcriptional programs and extrinsic modulation that are required for specification, positioning and wiring of different types of interneurons. The lab uses both in vivo and in vitro approaches, thus studying gene expression pattern in neurons during development in vivo and reproducing in vivo differentiation programs for generation of neuronal subtypes in vitro. Mechanisms of function for genes that regulate neuronal differentiation and maturation are studied in details in vivo in mouse models and in vitro in human cell cultures.