曹成喜 的个人介绍页
曹成喜
曹成喜
教授
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个人简历

上海交通大学教授、博导和首席科学家。2000年于中国科技大学化学系获理学博士学位,毕业后留校工作。2002年-现在,于上海交通大学生命科学技术学院工作。2006年澳大利亚La Trobe大学学术交流访问。主讲多门本科和研究生课程,包括《生物分离工程》、《生物分析化学》和《高等电泳与色谱技术》等。现任国家重大科学仪器设备开发专项首席科学家和技术专家委员会副主任、通用电泳设备国家标准委员会委员、中国化学会有机与生化分析专业委员会委员、第七-第九全国微纳生物化学分离分析会议学术委员会委员、TOACJ 期刊(Bentham Science Publishers Ltd出版)编委。曾荣获安徽省骨干教师、上海交通大学优秀教师和优秀科研团队称号荣誉等。应邀为英国Analyst (IF=3.9)、Electrophoresis (IF=3.6)、Anal Bioanal. Chem (IF=3.8) 和Anal Chim Acta (IF=4.3)等国际主流期刊撰写并发表评论性综述和论文等,多次在国际会议做邀请报告,应邀为Anal. Chem., Biosen Bioelectr, Electrophoresis, J. Chromatogr. A, Analyist, Anal. Chim Acta, Talanta和J. Chromatogr. B等十多种国际主流学术期刊担任特邀论文评议人。

先后主持20多项科研项目,包括首批国家重大科学仪器设备开发专项1项(首席科学家)、国家自然科学基金重点项目1项、面上等课题6项、国家973重大基础研究子课题1项、国家863重点支撑项目子课题1项、安徽省自然科学基金1项、卫生部课题1项、安徽省课题4项、横向课题6项。主编教材2部,包括《生化分析技术》和《生物化学 仪器分析基础》。先后在Anal Chem, J. Chromatogr A, Electrophoresis, Anal. Chim Acta, Talanta和J. Chromatogr. B等重要学术期刊发表论文90余篇(其中SCI收录论文70余篇),近几年获得或申请国家发明专利10多项。

研究方向

研究方向一:
重要生物技术一一电泳学新原理、新方法和应用研究。紧紧围绕我们实验室在国际上率先开拓的优势方向,开展移动反应界面(MRB) (包括移动亲和界面(MAB)、超分子界面(MSB)、中和反应界面(MNB)、络合反应界面(MCB)和亲和界面(MAB)等)新概念、新模型、新理论、新方法及其计算机模拟等的研究。


研究方向二:
制备性电泳分离技术的研究。结合重组蛋白分离纯化存在问题、以及实验室在自由流电泳(free-flow electrophoresis, FFE)和MRB等在国际上形成的技术优势,开展FFE分离富集新原理、新设备、新方法、及其在多种生物物质(包括蛋白质、药物、细胞、细胞器等)分离富集等方面的研究。


研究方向三:
双向凝胶电泳2DE成套设备技术开发与应用的研究。围绕生命科学重大研究需求,依托国家重大科学仪器设备开发专项,依靠实验室在2DE核心技术和关键部件方面的突破,研制具有自主知识产权的2DE成套仪器与技术,加速我国电泳仪器的产业升级。

研究方向四:
对接国家社会民生的重大需求,围绕重大疾病糖尿病等诊断问题,开发具有自主知识产权的糖尿病诊断新仪器、新技术和新试剂等,提升我国在糖尿病诊断等方面的技术与产业化水平,提高相关企业的核心竞争力。

发表论文

1. Y. P. Fan, S. Li,** L. Y. Fan, C. X. Cao,* Visual offline sample stacking via moving neutralization boundary electrophoresis for analysis of heavy metal ion, Talanta, in press, doi: 10.1016/j.talanta.2012.03.045. IF=3.7.
2. J. Shao, L.Y. Fan, C. X. Cao,* X. Q. Huang, Y. Q. Xu,** Quantitative investigation on resolution increase of free-flow electrophoresis via simple interval sample injection and separation, Electrophoresis, 2012, doi: 10.1002/elps.201200169. IF=3.6.
3. B. Pang, J. Shao1, J. Zhang, J.-Z. Geng, L.-Y. Fan,** C.-X. Cao,* J.-L. Hou, Enhancing separation of histidine from amino acids via free-flow affinity electrophoresis with gravity-induced uniform hydrodynamic flow, Electrophoresis, 2012, doi: 10.1002/elps. 20114130. IF=3.6.
4. J. Z. Geng, J. Shao, J. H. Yang, B. Pang, C X Cao,* L. Y. Fan**, Re-assemblable quasi-chip free-flow electrophoresis with simple heating dispersion for rapid micropreparation of trypsin in crude porcine pancreatin, Electrophoresis, 2011, 32, 3248–3256. IF=3.6
5. Q. Yang, L. Y. Fan, S. S. Huang**, W. Zhang, C X Cao*, Equivalence-point electro- migration acid-base titration via moving neutralization boundary electrophoresis, Electrophoresis, 2011, 32, 1015-1024. IF=3.6
6. C. X. Cao*, W. Zhang, L. Y. Fan**, Comparative study on sample stacking by moving reaction boundary formed with weak acid and weak or strong alkali in capillary electrophoresis: I. Theory, Talanta, 2011, 84, 558-567. IF = 3.7
7. W. Zhang, L. Y. Fan**, C. X. Cao*, Comparative study on sample stacking by moving reaction boundary formed with weak acid and weak or strong base in capillary electrophoresis: II. Experiments, Talanta, 2011, 84, 547-557. IF = 3.7
8. C. Sun, X. D. Yang, L. Y. Fan, W. Zhang, Y. Q. Xu, C. X. Cao*, Stacking and determination of phenazine-1-carboxylic acid with low pKa in soil via moving reaction boundary formed by alkaline and double-acidic buffers in capillary electrophoresis, Anal. Bioanal. Chem, (invited). 2011, 399, 3441-3450. IF = 3.8
9. J. Shao, L.-Y. Fan, W. Zhang, C. G. Guo, S. Li, Y. Q. Xu**, C.-X. Cao*, Purification of phenazine-1-carboxylic acid by Free-Flow Electrophoresis from fermentation broth of Pseudomonas M-18, Electrophoresis, 2010, 31, 3499-3507. IF = 3.6
10. J. Shao, C.-X. Cao*, S. Li, W. Zhang, L.-Y. Fan, R. Sun, Y.-C. Dong, Controlling of band width, resolution and sample loading by injection system in a simple preparative free-flow electrophoresis with gratis gravity J. Chromatogr. A, 2010, 1217, 2182-2186. IF = 4.2
11. J. Meng, W. Zhang, C. X. Cao*, L. Y. Fan, Moving affinity boundary electrophoresis and its exclusive capture of target metabolite in human urine, Analyst, 2010, 135 1592-1599. IF=3.9
12. W. Zhang, J. F. Chen, L.Y. Fan, C. X. Cao*, J.C. Ren, S. Li, J. Shao, A novel Isotachophoresis of Cobalt and Copper Complexes by Metal Ion Substitution Reaction in a Continuous Moving Chelation Boundary, Analyst, 2010, 135, 140-148. IF=3.9
13. S. Chen, J. F. Palmer, W. Zhang, J. Shao, S., Li, L. Y. Fan**, R. Sun, Y. C. Dong, C. X. Cao*, A Simple Preparative Free-flow Electrophoresis Joined with Gratis Gravity: I. Gas Cushion Injector and Self-balance Collector instead of Multiple Channel Pump, Electrophoresis, 2009, 30, 1998-2007. IF = 3.6
14. L.-Y. Fan, W. Yan, W. Zhang, Q. Chen, C. X. Cao*, Experiments on Moving Interaction Boundaries and Their Characteristics of Focusing and Probing of Both Guest and Host Target Molecules, Anal. Chim. Acta, 2009, 650, 111-117. (invited) IF = 4.3
15. W. Zhu, W. Zhang, L. Y. Fan**, J. Shao, S. Li, J. L. Chen, C. X. Cao*, Study on Mechanism of Stacking of Zwitterion in Highly Saline Biologic Sample by Transient Moving Reaction Boundary Created by Formic Buffer and Conjugate Base in Capillary Electrophoresis, Talanta, 2009, 78, 1194-1200. IF = 3.7
16. J. Jin, J. Shao, S. Li, W. Zhang, L.-Y. Fan, C.-X Cao*, Computer simulation on a continuous moving chelation boundary in EDTA-based sample sweeping in capillary electrophoresis, J. Chromatogr. A, 2009, 1216, 4913-4922. IF = 4.2
17. C. X. Cao, L. Y. Fan, W. Zhang, Review on the theory of moving reaction boundary, electromigration reaction methods and applications in isoelectric focusing and sample preconcentration, Analyst, (invited), 2008, 133, 1139-1157. IF = 3.9
18. Q. Chen, L. Y. Fan, W. Zhang, C. X. Cao, Separation and determination of abused drugs clenbuterol and salbutamol from complex extractants in swine feed by capillary zone electrophoresis with simple pretreatment, Talanta, 2008, 76, 282-287. IF = 3.7
19. H. M. Liu, X. H. Zhang, X. Q. Huang XQ, C. X. Cao, Y. Q. Xu, Rapid quantitative analysis of phenazine-1-carboxylic acid and 2-hydroxyphenazine from fermentation culture of Pseudomonas chlororaphis GP72 by capillary zone electrophoresis, Talanta, 2008, 76, 276-281. IF = 3.7
20. L. Y. Fan, C. J. Li, W. Zhang, C. X. Cao, P. Zhou, Z. X. Deng, Moving Chelation Boundary: Model, Theory and Its Controllable Metal Ionic Stacking Efficiency in Capillary Electrophoresis. Electrophoresis, 2008, 29, 3989-3998. IF = 3.6
21. X. Zhou, L. Y. Fan, W. Zhang, C. X. Cao, Separation and determination of acrylamide in potato chips by micellar electrokinetic capillary chromatography, Talanta, 2007, 71, 1541-1545. IF = 3.7
22. M. Li, L. Y. Fan, W. Zhang, C. X. Cao, Stacking and quantitative analysis of lovastatin in urine sample by transient moving chemical reaction boundary method in capillary electrophoresis, Anal. Bioanal. Chem., 2007, 387, 2719-2725. IF = 3.9
23. X. Wang, W. Zhang, L. Y. Fan, C. X. Cao, Quantitative detection of matrine and oxymatrine in rate plasma by capillary electrophoresis with moving reaction boundary-based sample stacking, Anal. Chim. Acta, 2007, 594, 290-296. IF = 4.3
24. Q. L. Wang, L. Y. Fan, W. Zhang, C. X. Cao, Sensitive analysis of two barbiturates in human urine by capillary electrophoresis with sample stacking induced by moving reaction boundary, Anal. Chim. Acta, 2006, 580, 200-205. IF = 4.3
25. C.-X. Cao, W. Zhang, W.-H. Qin, S. Li, W. Liu, Quantitative predictions to conditions of zwitterionic stacking by moving chemical reaction boundary created with weak electrolytic buffers in capillary zone electrophoresis, Anal. Chem., 2005; 77, 955-963. IF = 5.8
26. W.-H. Qin, C.-X. Cao, W. Zhang, S. Li, W. Liu, Quantitative predictions to selective stacking of zwitterions by moving chemical reaction boundary in capillary electrophoresis, Electrophoresis, 2005, 26, 3113-3124. IF = 3.6
27. C.-X. Cao, Y.-Z. He, M. Li, Y.-T. Qian, M.-F. Gao, L.-H. Ge, S.-L. Zhou, L. Yang and Q.-S. Qu, Stacking ionizable analytes in a sample matrix with high salt by a transient moving chemical reaction boundary, method in capillary zone electrophoresis Anal. Chem., 2002; 74: 4167-4174. IF = 5.8
28. C.-X. Cao, S.-L. Zhou, Y.-T. Qian, Y.-Z. He, C.-R. Wang, L. Yang, Q.-S. Qu and W.-K. Chen, Investigations on factors that influence the moving neutralization reaction boundary method for capillary electrophoresis and isoelectric focusing J. Chromatogr. A, 2002; 952: 29-38. IF = 4.2
29. C.-X. Cao, Y.-Z. He, M. Li, Y.-T. Qian, L. Yang, Q.-S. Qu, S.-L. Zhou and W.-K. Chen, Improving separation efficiency of capillary zone electrophoresis of tryptophan and phenylalanine with the transient moving chemical reaction boundary method, J. Chromatogr. A, 2002; 952: 39-46. IF = 4.2
30. C.-X. Cao, S.-L. Zhou, Y.-Z. He, X.-Y. Zheng, W.-K. Chen and Y.-T. Qian, Corrections to Moving Chemical Reaction Boundary Equation(MCRBE) for weak Reactive Electrolyte System With Background electrolyte KCl in Large Concentration, J. Chromatogr. A, 2001; 907: 347-352. IF = 4.2
31. Ca C.-X., Zhou S.-L., Qian Y.-T., He Y.-Z., Yang L., Qu Q.-S., Chen W.-K., Experimental investigation on moving chemical reaction boundary theory for weak-acid--strong-base system with background electrolyte KCl in large concentration. J. Chromatogr. A, 2001; 922: 283-292. IF = 4.2
32. C.-X. Cao, S.-L. Zhou, Y.-Z. He, X.-Y. Zheng, W.-K. Chen and Y.-T. Qian, Experimental Study on Moving Neutralization Reaction Boundary Created with the Strong Reactive Electrolytes of HCl and NaOH in Agarose Gel, J. Chromatogr. A, 2000; 891: 337-347. IF = 4.2
33. C.-X. Cao, Q.-S.Zheng, R.-Z.Li, and J.-H.Zhu The un-validity of Kohlrausch' regulating function for Svensson's isoelectric focusing and stationary electrolysis at steady-state. J. Chromatogr. A, 1999; 863: 219-226. IF = 4.2
34. C.-X. Cao, Moving chemical reaction boundary and isoelectric focusing: I the conditional equations of Svensson-Tiselius' differential equation of solute concentration distribution in an idealized isoelectric focusing at steady state, J. Chromatogr. A, 1998; 813: 153-172. IF = 4.2
35. C.-X. Cao, Moving chemical reaction boundary and isoelectric focusing: II the existence of (or quasi-) equal-fluxes(or transference-numbers) of proton and hydroxyl ion in stationary electrolysis and Svensson's isoelectric focusing, J. Chromatogr. A, 1998; 813: 173-177. IF = 4.2
36. C.-X. Cao, Comparisons between the mobilities of small salt ions obtained by moving boundary method and two empirical equations in capillary electrophoresis, J. Chromatogr. A, 1997; 771: 375-378. IF = 4.2
37. C.-X. Cao, Mobilities of proton obtained by moving boundary method and an empirical equation in capillary electrophoresis, J. High. Resol. Chromatogr., 1997; 20, 701-702. IF = 4.1

研究成果

主要学术贡献:
1、提出了较系统的电泳学新概念、新理论和新方法——移动反应界面(MRB),包括亲和反应界面、相互作用界面、中和反应界面、沉淀反应界面、络合反应界面和氧化还原反应界面等,建立了一系列MRB的方法,验证了MRB概念、理论和方法;
2、发展了系统的基于MRB的等电聚焦电泳(IEF)动力学理论,解决了50年来一直存在于IEF和2DE的五个基础科学问题,合成了IEF和2DE关键部件,为IEF和2DE的进一步发展与应用奠定了重要基础;
3、利用MRB,发展了多种毛细管电泳在线富集技术的理论和方法,并为其他富集技术的阐明提供了基础;提出了大体积样本同步富集分离的新理论和新方法,发展了基于MRB的离线电泳富集技术与装置;
4、建立了基于MRB的电泳滴定原理、技术和方法,研制了国际上第一台MRB电泳滴定原始样机,初步建立了蛋白质滴定的原理和技术,提出了基于MRB的电泳阻滞信号及其生化传感器的概念;
5、研制了国际上第一台重力自平衡-自由流电泳FFE装置(包括大型、中型和芯片级FFE设备),研究了相关的进样技术、区带控制技术、重要生物物质(细胞、蛋白、酶和抗生素等)分离技术;
6、建立了多种MRB、毛细管区带电泳(CZE)和自由流电泳(FFE)的数学模型和计算机模拟软件等,利用计算机模拟结果揭示了经典电泳理论omega函数(具有100多年历史)与MRB理论之间的关系。

正在开展的课题
(1)双向凝胶电泳2DE成套设备技术开发与应用 首批国家重大仪器专项
(2)基于MRB的复杂蛋白质分离分析新原理和新方法的研究 NSFC重点项目
(3)糖尿病诊断新仪器、新技术与关键部件研制 首批国家重大仪器专项
(4)微生物及其代谢物分离新电泳设备的研制与应用 科技部973重大研究计划
(5)基于MRB的稳定、快速、高通量诊断仪器的研制 医工(理)交叉重点基金

成果与专利
No. 著作权/专利名称 (状态) 申请号/授权号
1 毛细管区带电泳模拟软件V1.0 (授权) 2007SR17276
2 移动络合界面的模拟软件V1.0 (授权) 2010SR016459
3 用于毛细管电泳的电动近样符合管 (授权) Zl200410018026.5
4 用于自由流电泳的分离室缓冲分流装置 (授权) Zl200510024412.x
5 用于自由流电泳的分离液自平衡连通收集装置 (授权) Zl200510024413
6 自由流电泳分离室 (授权) Zl200710053315.x
7 用于研究等电聚焦电泳动力学的装置 (授权) Zl200610025466.2
8 一种用于多肽兴奋剂检测的等电聚焦电泳装置 (授权) Zl200610024274.x
9 用于等电聚焦电泳的微型毛细管阵列装置 (公开) 201010564747.1
10 用于测定酸碱浓度的电迁移酸碱滴定装置 (公开) 201010596012.7
11 青霉烯酸醇汞盐及其制备方法 (公开) 200910056685.0
12 铜离子半抗原青霉烯酸硫醇铜盐及其制备方法 (公开) 201010186178.1
12 镉离子半抗原青霉烯酸硫醇铜盐及其制备方法 (公开) 201110080342.5