刘建华 的个人介绍页
刘建华
刘建华
教授
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34204192

个人简历

教授,博士生导师。1989年中国科学院上海生物化学研究所研究生毕业,获博士 学位。1990年赴美国加州大学欧文分校(UC Irvine)医学院生化系做博士后。1993年回国后,在中科院上海生化所,中科院上海生命科学研究中心工作,任副研究员、真核基因表达调控研究组组 长。1998年调入上海交通大学生命科学技术学院任教授。中国生化与分子生物学会会员。主持完成5项国家自然科学基金,2项国家863项目,3项国家973二级子项目,多项上海市科委项目;合作完成多项国家自然科学基金重点项目等。已总结40余篇SCI论文,获得授权了10多项国家发明专利。

研究方向

1) 极端微生物的DNA损伤修复和DNA复制机制
2) 核酸检测与基因多态性分型
3) 微生物基因改造与功能研究
4) 药用蛋白制造和功能研究

发表论文

[1] Lu Zheng, Liang Rubing, Liu Xipeng, Hou Jingli, Liu Jianhua RNase HIII from Chlamydophila pneumoniae can efficiently cleave double-stranded DNA carrying a chimeric ribonucleotide in the presence of manganese. Mol Microbiol. 2012 Mar; 83(5):1080-1093.
[2] Liu Xipeng, Liu Jianhua Characterization of family IV UDG from Aeropyrum pernix and its application in hot-start PCR by family B DNA polymerase, 2011 Nov 8, PLoS One. 6(11): e27248.
[3] Rubing Liang, Jing Zhou, Wenhua Wang, Jianhua Liu. Construction of an estrogen detection bacteria assay based on an estrogen-sensitive intein. Applied and Environmental Microbiology, 2011, 77(7): 2488-2495.
[4] Rubing Liang, Jianhua Liu. Scarless and sequential gene modification in Pseudomonas using PCR product flanked by short homology regions. BMC Microbiology, 2010, 10 (1): 209
[5] Liu Xipeng, Liu Jianhua. The terminal 5' phosphate and proximate phosphorothioate promote ligation-independent cloning. Protein Sci. 2010 May; 19(5): 967-973.
[6] Liu Xipeng, Hou Jingli, Liu Jianhua. A novel single nucleotide polymorphism detection of a double-stranded DNA target by a ribonucleotide-carrying molecular beacon and thermostable RNase HII. Anal Biochem. 2010 Mar 1; 398(1):83-92.
[7] Liu Xipeng, Li Chunpeng, Hou Jingli, Liu Yufen, Liang Rubing, Liu Jianhua. Expression and characterization of thymine-DNA glycosylase from Aeropyrum pernix. Protein Expr Purif. 2010 Mar; 70(1):1-6.
[8] Rubing Liang, Jianhua Liu. In-frame deletion of Escherichia coli essential genes in complex regulon. BioTechniques, 2008, 44 (2): 209-215
[9] Liu Xipeng, Zhang Yang, Liang Rubing, Hou Jingli, Liu Jianhua. Characterization of the 3' exonuclease of Chlamydophila pneumoniae endonuclease IV on double-stranded DNA and the RNA strand of RNA/DNA hybrid. Biochem Biophys Res Commun. 2007 Oct 5; 361(4):987-993.
[10] Liang Rubing, Liu Xipeng, Liu Jianhua, Ren Qiushi, Liang Peiji, Lin Zhixin, Xie Xiangming. A T7-expression system under temperature control could create temperature-sensitive phenotype of target gene in Escherichia coli. J Microbiol Methods. 2007 Mar; 68(3): 497-506.
[11] Liang Rubing, Liu Xipeng, Pei Dongli, Liu Jianhua. Biochemical characterization and functional complementation of ribonuclease HII and ribonuclease HIII from Chlamydophila pneumoniae AR39. Microbiology(UK). 2007 Mar; 153(Pt 3):787-793.
[12] Hou Jingli, Liu Xipeng, Pei Dongli, Liu Jianhua. RNase HII from Chlamydia pneumoniae discriminates mismatches incorporation into DNA-rN1-DNA/DNA duplexes. Biochem Biophys Res Commun. 2007 May 18; 356(4):988-992.
[13] Hou Jingli, Liu Xipeng, Zheng Yan, Liu Jianhua, DuanTao. Molecular beacons for isothermal fluorescence enhancement by the cleavage of RNase HII from Chlamydia pneumoniae. Anal Biochem. 2007 Dec 15; 371(2):162-166.
[14] Hou Jingli, Liu Xipeng, Zheng Yan, Liu Jianhua. A Method for HLA Genotyping Using the Specific Cleavage of DNA-rN1-DNA/DNA with RNase HII from Chlamydia pneumoniae. Oligonucleotides, 2007 Winter, 17(4):433-444.
[15] Liu Xipeng, Hou Jingli, Liu Jianhua. Chlamydial DNA polymerase I can bypass lesions in vitro. Biochem Biophys Res Commun. 2006 Jul 7; 345(3):1083-1091.
[16] Liu Xipeng, Liu Jianhua. Chlamydia pneumoniae AP endonuclease IV could cleave AP sites of double- and single-stranded DNA. Biochim Biophys Acta. 2005 Dec 1; 1753(2):217-225.
[17] Liu Xipeng, Liu Jianhua. The mechanism of base excision repair in Chlamydiophila pneumoniae. DNA Repair 2005 Nov 21; 4(11):1295-1305.
[18] Jing Yu, Xiaomin Yu, Jianhua Liu. A thermostable manganese-containing superoxide dismutase from pathogen Chlamydia pneumoniae.FEBS Lett. 2004, 562(1-3):22-6.

研究成果

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