周宁一 的个人介绍页
周宁一
周宁一
特聘教授
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个人简历

上海交通大学特聘教授,生命学院微生物科学系系主任,微生物代谢国家重点实验室分子环境微生物研究组组长。获武汉大学微生物学专业学士和英国伦敦帝国理工学院(Imperial College London)微生物学专业博士学位,并在帝国理工学院和威尔士大学(University of Wales, Bangor)从事博士后研究。中国科学院"百人计划"入选者,曾任中国科学院武汉病毒研究所研究员,中国科学院农业与环境微生物重点实验室主任。目前兼任中国微生物学会常务理事,中国微生物学会环境微生物学专业委员会主任,《微生物通报》副主编,Editor for Applied and Environmental Microbiology, Associate Editor for Frontiers in Microbiology。主要从事微生物分解代谢芳香烃污染物的生理、生化和分子生物,以及污染土壤的微生物修复等方面的研究。获"国务院政府特殊津贴", "中国科学院朱李月华优秀教师奖"等荣誉和奖励。2014-2016连续三年入选爱思唯尔(Elsevier)发布的中国高被引学者榜单(“免疫与微生物学”领域)

研究方向

主要从事微生物分解代谢芳香烃污染物的生理、生化和分子生物,以及污染土壤的微生物修复等方面的研究。研究领域涉及微生物降解芳香烃的代谢途径解析及其调控,芳烃化合物的转运,催化代谢途径中反应的酶学,合成生物学在构建降解工程菌的应用,污染土壤的微生物修复机理及其分子生态学等。主持了国家自然科学基金重点项目、863项目、973课题等10余项国家级项目,在国际学术刊物上发表论文60余篇。获发明专利授权6项。

发表论文

Representative publications (*corresponding author):
1. Gao YZ , Liu H, Chao HJ, and Zhou NY*, 2016. Constitutive expression of Nag-like dioxygenase gene through an internal promoter in the 2-chloronitrobenzene catabolic gene cluster of Pseudomonas stutzeri ZWLR2-1. Appl. Environ. Microbiol. 82:3461-3470.
2. Min J, Zhang JJ, Zhou NY*. 2016. A Two-Component para-nitrophenol monooxygenase initiates a novel 2-chloro-4-nitrophenol catabolism pathway in Rhodococcus imtechensis RKJ300. Appl. Environ. Microbiol. 82: 714-723.
3. Chao HJ, Chen YF, Fang T, Xu Y, Huang WE, Zhou NY*. 2016. HipH catalyzes the hydroxylation of 4-hydroxyisophthalate to protocatechuate in 2, 4-xylenol catabolism by pseudomonas putida NCIMB 9866. Appl. Environ. Microbiol. 82: 724-731.
4. Liu S, Su T, Zhang C, Zhang, WM, Zhu D, Su J, Wei T, Wang K, Huang Y, Guo L, Xu S, Zhou NY*, Gu L*. 2015. Crystal structure of PnpCD, a two-subunit hydroquinone 1, 2-dioxygenase, reveals a novel structural class of Fe2+-dependent dioxygenases. J Biol Chem, 290: 24547-24560.
5. Liu K Xu Y, Zhou NY*. 2015. Identification of a specific maleate hydratase in the direct hydrolysis route of the gentisate pathway. Appl. Environ. Microbiol. 81: 5753-5760
6. Zhang WM, Zhang JJ, Jiang X, Chao HJ, Zhou NY*. 2015. Transcriptional activation of multiple operons involved in para-nitrophenol degradation by Pseudomonas sp. strain WBC-3. Appl. Environ. Microbiol. 81: 220-230
7. Min J, Zhang JJ, and Zhou NY*. 2014. The gene cluster for para-nitrophenol catabolism is responsible for 2-chloro-4-nitrophenol degradation in Burkholderia sp. strain SJ98. Appl. Environ. Microbiol. 80: 6212-6222
8. Chao HJ, Zhou NY*. 2014. Involvement of the global regulator GlxR in 3-hydroxybenzoate and gentisate utilization by Corynebacterium glutamicum. Appl. Environ. Microbiol. 80:4215-4225
9. Xu Y, Chen B, Chao HJ, Zhou NY*. 2013. mhpT encodes an active transporter involved in 3-(3-hydroxyphenyl)propionate catabolism by Escherichia coli K-12. Appl. Environ. Microbiol. 79: 6362-6368
10. Sun DL, Jiang X, Wu QL, Zhou NY*. 2013. Intragenomic heterogeneity of 16S rRNA genes causes overestimation of prokaryotic diversity. Appl. Environ. Microbiol. 79:5962-5969
11. Chao HJ, Zhou NY*. 2013. GenR, an IclR-type regulator, activates and represses the transcription of gen genes involved in 3-hydroxybenzoate and gentisate catabolism in Corynebacterium glutamicum. J. Bacteriol. 195: 1598-1609
12. Liu K, Liu TT, Zhou NY*. 2013. HbzF catalyzes direct hydrolysis of maleylpyruvate in the gentisate pathway of Pseudomonas alcaligenes NCIMB 9867. Appl. Environ. Microbiol. 79: 1044-1047
13. Chi XQ, Zhang JJ, Zhao S, Zhou NY*. 2013. Bioaugmentation with a consortium of bacterial nitrophenol-degraders for remediation of soil contaminated with three nitrophenol isomers. Environ. Pollut.172:33-41
14. Liu TT , Zhou NY*. 2012. Novel L-cysteine dependent maleylpyruvate isomerase in the gentisate pathway of Paenibacillus sp. strain NyZ101. J. Bacteriol. 194:3987-3994
15. Xiao Y, Liu TT, Dai H, Zhang JJ, Liu H, Tang H, Leak DJ, Zhou NY*. 2012. OnpA, an unusual flavin dependent monooxygenase containing a cytochrome b5 domain. J. Bacteriol. 194: 1342-1349
16. Xu Y, Gao X, Wang SH, Liu H, Williams PA, Zhou NY*. 2012. MhbT is a specific transporter for 3-hydroxybenzoate uptake by Gram-negative bacteria. Appl. Environ. Microbiol. 78:6113-6120
17. Liu H, Wang SJ, Zhang JJ, Dai H, Tang H, Zhou NY*. 2011. Patchwork assembly of nag-like nitroarene dioxygenase genes and 3chlorocatechol degradation cluster for the pathway evolution of 2-chloronitrobenzene catabolism in Pseudomonas stutzeri ZWLR21. Appl. Environ. Microbiol. 77: 4547-4552
18. Zhang JJ, Liu H, Xiao Y, Zhang XE, Zhou NY*. 2009. Identification and characterization of catabolic para-nitrophenol 4-monooxygenase and para-benzoquinone reductase from Pseudomonas sp. strain WBC3. J. Bacteriol. 191:2703-2710
19. Zhao S, Ramette A, Niu GL, Liu H, Zhou NY*. 2009. Effects of nitrobenzene contamination and of bioaugmentation on nitrification and ammonia-oxidizing bacteria in soil. FEMS Microbiol. Ecol. 70:315-323
20. Niu GL, Zhang JJ, Zhao S, Liu H, Boon N, Zhou NY*. 2009. Bioaugmentation of a 4-chloronitrobenzene contaminated soil with Pseudomonas putida ZWL73. Environ. Pollut. 157:763-771.
21. Xiao Y, Zhang JJ, Liu H, and Zhou NY*. 2007. Molecular characterization of a novel ortho-nitrophenol catabolic gene cluster in Alcaligenes sp. strain NyZ215. J.Bacteriol.189: 6587-6593.
22. Yan DZ, Liu H, Zhou NY*. 2006. Conversion of Sphingobium chlorophenolicum ATCC 39723 to a hexachlorobenzene degrader by metabolic engineering. Appl. Environ. Microbiol. 72:2283-2286
23. Zhou, NY, Fuenmayor SL, Williams PA*. 2001. nag genes of Ralstonia (formerly Pseudomonas) sp. strain U2 encoding enzymes for gentisate catabolism. J. Bacteriol. 183:700-708.
24. Zhou, NY, Al-Dulayymi J, Baird MS, Williams PA*. 2002. Salicylate 5-hydroxylase from Ralstonia sp. strain U2: a monooxygenase with close relationships to and shared electron transport proteins with naphthalene dioxygenase. J. Bacteriol. 184:1547-1555.
25. Zhou, NY, Jenkins A, Chan Kwo Chion C K, Leak DJ*. 1999. The alkene monooxygenase from Xanthobacter strain Py2 is closely related to aromatic monooxygenases and catalyzes aromatic monohydroxylation of benzene, toluene, and phenol. Appl. Environ. Microbiol.65:1589-1595.

研究成果

1. 揭示了微生物分解代谢单硝基酚三种同分异构体的机理(JB 189:6587, JB 191:2703, JB 194: 134), 2-氯-4-硝基酚的代谢(AEM 80: 6212),4-硝基酚代谢的转录调控(AEM 81: 220),和2-氯硝基苯的分解代谢途径的进化(AEM 77: 4547);
2. 发现了龙胆酸代谢途径中依赖于半胱氨酸的顺丁烯二酸单酰丙酮酸异构酶(JB 194:3987) 和顺丁烯二酸单酰丙酮酸水解酶(AEM 79: 1044)并揭示了龙胆酸代谢的调控机理(JB 195: 1598,AEM 80:4215);
3. 发现并鉴定了微生物3-羟基苯甲酸(AEM 78:6113)和3-羟基苯丙酸(AEM 79: 6362)的转运蛋白;
4. 利用代谢工程成功地构建了降解六氯苯[持久性有机污染物(POPs)之一]的工程菌(AEM 72:2283);
5. 生物修复硝基苯(FEMS Microbiol. Ecol. 70:315),4-氯硝基苯(Environ. Pollut. 157:763)和单硝基酚三种同分异构体(Environ. Pollut.172:3)的污染土壤并揭示其相关分子生态学效应。
6. 阐明了原核生物基因组内16SrRNA基因的异质性会引起其多样性的高估(AEM 79:5962).