钱志刚 的个人介绍页
钱志刚
钱志刚
副研究员
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34207248

个人简历

2001年7月毕业于华东理工大学,获得生物化学工程专业学士学位;2004年3月毕业于华东理工大学,获得生物化工硕士学位;2009 年8月毕业于韩国科学技术院(KAIST),获得生命化学工学科博士学位。2009年8月至2010年8月在Sang Yup Lee教授实验室从事博士后工作,继续研究微生物系统代谢工程生物合成化学品和蛋白材料。2010 年11月至2012 年4月在美国波士顿大学生物医学工程系进行博士后研究,合作导师为James J. Collins教授,主要研究合成生态系统。2012年5月回国任上海交通大学生命科学技术学院副研究员,主要从事蛋白质工程、系统代谢工程与合成生物学的交叉学科研究。在包括PANS 等重要国际学术刊物上发表研究论文13篇。所发表的论文被选为Nature 杂志的Research Highlight,Nature Chemical Biology杂志的News and Views,Biotechnology and Bioengineering杂志的封面、亮点文章和Editors’Choice。申请或获得发明专利12项,其中包括3项国际专利。

研究方向

Protein engineering
Synthetic biology
Systems metabolic engineering

发表论文

INTERNATIONAL JOURNAL
13. Xia XX, Qian ZG, Lee SY. 2011. Comparative proteomic and genetic analyses reveal unidentified mutations in Escherichia coli XL1-Blue and DH5α. FEMS Microbiol Lett 314(2):119-124.

12. Qian ZG, Xia XX, Lee SY. 2011. Metabolic engineering of Escherichia coli for the production of cadaverine: A five carbon diamine. Biotechnol Bioeng 108(1): 93-103.

11. Xia XX*, Qian ZG*, Ki, CS, Park, YH, Kaplan, DL, Lee SY. 2010. Native-sized recombinant spider silk protein produced in metabolically engineered Escherichia coli results in a strong fiber. Proc Natl Acad Sci USA 107(32):14059-14063. (Xia and Qian contributed equally).
News and Views in Nature Chemical Biology (Linke WA. Biomaterials: Spider strength and stretchability. Nature Chemical Biology 6:702-703; October 2010)
Featured by MIT Technology Review (3 August 2010)
Featured by TCE Today (September 2010)

10. Qian ZG, Xia XX, Lee SY. 2009. Metabolic engineering of Escherichia coli for the production of putrescine: A four carbon diamine. Biotechnol Bioeng 104(4):651-662. Editors' Choice, Journal cover paper
Wiley-Blackwell Press Release ‘Pioneering research succeeds in producing industrially vital chemical through engineered bacteria’ (27 August 2009); in numerous science news web sites
Nature Research Highlight ‘Bacterial factories’ (Nature 461:14-15; 3 September 2009)
BTJ-FORUM ‘Metabolic engineering of E. coli’ (Biotechnology Journal, 4:1376; October 2009)
Top 25 Most Downloaded Articles From September-December 2009 (http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-0290/homepage/most_accessed_2009.htm)

9. Qian ZG, Xia XX, Choi JH, Lee SY. 2008. Proteome-based identification of fusion partner for high-level extracellular production of recombinant proteins in Escherichia coli. Biotechnol Bioeng 101(3):587-601.
Spotlight ‘A proteome-based approach to identifying fusion partner for recombinant protein excretion’ (Biotechnology and Bioengineering DOI: 10.1002/bit.22096; 15 October 2008)

8. Xu Y, Zhao Z, Qian X, Qian Z, Tian W, Zhong J. 2006. Novel, unnatural benzo-1,2,3-thiadiazole-7-carboxylate elicitors of taxoid biosynthesis. J Agric Food Chem 54(23):8793-8798.

7. Qian ZG, Zhao ZJ, Xu Y, Qian X, Zhong JJ. 2006. Novel synthetic 2,6-dichloroisonicotinate derivatives as effective elicitors for inducing the biosynthesis of plant secondary metabolites. Appl Microbiol Biotechnol 71(2):164-167.

6. Qian ZG, Zhao ZJ, Xu Y, Qian X, Zhong JJ. 2006. Novel chemically synthesized salicylate derivative as an effective elicitor for inducing the biosynthesis of plant secondary metabolites. Biotechnol Prog 22(1):331-333.

5. Qian ZG, Zhao ZJ, Xu Y, Qian X, Zhong JJ. 2005. A novel synthetic fluoro-containing jasmonate derivative acts as a chemical inducing signal for plant secondary metabolism. Appl Microbiol Biotechnol 68(1):98-103.

4. Qian ZG, Zhao ZJ, Xu Y, Qian X, Zhong JJ. 2005. Highly efficient strategy for enhancing taxoid production by repeated elicitation with a newly synthesized jasmonate in fed-batch cultivation of Taxus chinensis cells. Biotechnol Bioeng 90(4):516-521.

3. Zhao Z, Xu Y, Qian Z, Tian W, Qian X, Zhong JJ. 2004. Novel fluoro- and hydroxyl-containing jasmonate derivatives as highly efficient elicitors in suspension cultures of Taxus chinensis. Bioorg Med Chem Lett 14(18):4755-4758.

2. Qian ZG, Zhao ZJ, Xu Y, Qian X, Zhong JJ. 2004. Novel chemically synthesized hydroxyl-containing jasmonates as powerful inducing signals for plant secondary metabolism. Biotechnol Bioeng 86(7):809-816.

1. Qian ZG, Zhao ZJ, Tian WH, Xu Y, Zhong JJ, Qian X. 2004. Novel synthetic jasmonates as highly efficient elicitors for taxoid production by suspension cultures of Taxus chinensis. Biotechnol Bioeng 86(5):595-599.

ORAL PRESENTATIONS
4. Qian ZG. Metabolic engineering of microbial cell factories for bioproduction of materials and chemicals. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, Nov. 21, 2011.

3. Qian ZG, Xia XX, Lee SY. A systematic proteome-based approach for high-level extracellular production of recombinant proteins in E. coli. The Korean Society for Microbiology and Biotechnology (KMB) international symposium & annual meeting. June 25-26, 2009, Daejeon, Korea.

2. Qian ZG, Xia XX, Choi JH, Lee SY. High-level extracellular production of recombinant proteins in Escherichia coli: a proteome-based approach. Korean Society for Biotechnology and. Bioengineering (KSBB) international symposium & annual meeting, October 18-19, 2007, Daegu, Korea.

1. Qian ZG, Yu KW, Hahn EJ, Paek KY. Towards efficient production of useful plant secondary metabolites by the use of newly chemically synthesized elicitors. Conference for the Korean Society for Horticultural Science, May 26-27, 2005, Jeju, Korea.

INTERNATIONAL POSTER PRESENTATIONS
20. Choi S, Qian ZG, Xia XX, Lee SY. Metabolic engineering of Escherichia coli for the production of cadaverine: A five carbon diamine. Society for Industrial Microbiology Annual Meeting and Exhibition, July 24-28, 2011, New Orleans, LS, USA.

19. Choi S, Qian ZG, Xia XX, Lee SY. Metabolic engineering of Escherichia coli for the efficient production of putrescine: A four carbon linear chain diamine. Society for Industrial Microbiology Annual Meeting and Exhibition, July 24-28, 2011, New Orleans, LS, USA.

18. Lee KY, Xia XX, Qian ZG, Lee SY. Production of large spider dragline silk proteins in metabolically engineered Escherichia coli to spin into a native quality silk fiber. Biochemical and Molecular Engineering XVII, June 26-30, 2011, Seattle, Washington, USA.

17. Lee KY, Qian ZG, Xia XX, Lee SY. Systems metabolic engineering of Escherichia coli for the efficient production of putrescine: A four carbon linear chain diamine. Biochemical and Molecular Engineering XVII, June 26-30, 2011, Seattle, Washington, USA.

16. Kim MY, Qian ZG, Xia XX, Lee SY. Engineering of Escherichia coli for the production of putrescine: A four carbon diamine. The 23rd International Symposium on Chmical Engineering, December 4-5, 2010, Kyushu Sangyo University, Fukuoka, Japan.

15. Lee SY, Qian ZG, Xia XX. Metabolic engineering of Escherichia coli for the production of 1,4-diaminobutane. 11th international symposium on the genetics of industrial microorganisms (GIM), June 28-July 1, 2010, Melbourne, Australia.

14. Lee SY, Qian ZG, Xia XX. A systematic proteome-based approach for high-level extracellular production of recombinant proteins in Escherichia coli. 11th international symposium on the genetics of industrial microorganisms (GIM), June 28-July 1, 2010, Melbourne, Australia.

13. Qian ZG, Xia XX, Lee SY. Systems metabolic engineering of Escherichia coli for the production of putrescine, a four carbon linear chain diamine. Metabolic Engineering VIII: Metabolic engineering for green growth. June 13-17, 2010, Jeju Island, Korea.

12. Qian ZG, Xia XX, Lee SY. Metabolic engineering of Escherichia coli for the production of large spider dragline silk proteins. Metabolic Engineering VIII: Metabolic engineering for green growth. June 13-17, 2010, Jeju Island, Korea.

11. Xia XX, Qian ZG, Lee SY. Comparative proteomic analysis of four biotechnologically important Escherichia coli strains for rational host selection. Asia Pacific Biochemical Engineering Conference (APBioChEC'09), November 24-27, 2009, Kobe, Japan.

10. Qian ZG, Xia XX, Lee SY. A systematic approach for high-level extracellular production of recombinant proteins in Escherichia coli. Asia Pacific Biochemical Engineering Conference (APBioChEC'09), November 24-27, 2009, Kobe, Japan.

9. Qian ZG, Xia XX, Lee SY. Systematic proteome-based approach for high-level extracellular production of recombinant proteins in Escherichia coli. The 16th Biochemical Engineering, July 5-9, 2009, Burlington, Vermont, USA.

8. Qian ZG, Xia XX, Choi JH, Lee SY. Systematic proteome-based approach for high-level extracellular production of recombinant proteins in Escherichia coli. 13th International Biotechnology Symposium & Exhibition (IBS2008), October 12-17, 2008, Dalian, China.

7. Xia XX, Qian ZG, Han MJ, Lee SY. The extracellular proteomes of Escherichia coli B and K-12 strains and its application in the secretory production of recombinant proteins. 13th International Biotechnology Symposium & Exhibition (IBS2008), October 12-17, 2008, Dalian, China.

6. Qian ZG, Xia XX, Choi JH, Lee SY. A systematic proteome-based approach for the secretion of synthetic gene products in Escherichia coli. Synthetic Biology 4.0 (SB4.0), October 10-12, 2008, Hong Kong, China.

5. Qian ZG, Xia XX, Choi JH, Lee SY. High-level extracellular production of recombinant proteins in Escherichia coli: a proteome-based approach. Asia Pacific Biochemical Engineering Conference (APBioChEC'07), November 4-7, 2007, Taipei, Taiwan.

4. Xia XX, Qian ZG, Han MJ, Choi JH, Lee SY, Yoo JS. The extracellular proteomes of Escherichia coli B and K-12 strains and their application in the excretory production of recombinant proteins. Biochemical Engineering XV, July 15-19, 2007, Quebec City, Canada.

3. Choi JH, Qian ZG, Lee SY. Development of extracellular production systems of recombinant proteins in recombinant Escherichia coli. The 4th recombinant protein production meeting: a comparative view on host physiology, September 21-23, 2006, Barcelona, Spain.

2. Choi JH, Qian ZG, Jang SH, Lee SY. Extracellular production of recombinant proteins. 10th international symposium on the genetics of industrial microorganisms (GIM), June 24-28, 2006, Prague, Czech Republic.

1. Qian ZG, Zhao ZJ, Tian WH, Xu Y, Zhong JJ, Qian X. Novel chemically-synthesized elicitors for overproduction of secondary metabolites by plant cells. The 9th Symposium of Young Asian Biochemical Engineers' Community (YABEC 2003), November 13-15, 2003, Jeju Island, Korea.

研究成果

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