贺新义 的个人介绍页
贺新义
贺新义
研究员
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mml.sjtu.edu.cn/
62932943

个人简历

研究兴趣一直聚焦于核苷酸代谢,包括基础代谢中DNA磷硫酰化,羟甲基化,糖羟甲基化等异常修饰形成和识别的分子机理;以及次级代谢中具有良好生物活性的核苷类抗生素的生物合成,如杀稻瘟菌素,米多霉素和精霉素的生物合成的酶学研究。近五年来已发表第一或通讯作者SCI论文14篇,合作论文发表在《自然》杂志上并被评为2011年度中国科学十大进展;获得授权发明专利3项;先后主持4项国家自然科学基金项目。2004、05、09、10和14年度上海交通大学教师年终考核优秀;2005年获中国高等学校十大科技进展;2010年度获得上海交通大学晨星青年学者奖励计划A类。

研究方向

1.细菌核苷酸修饰及识别的酶学研究
2.核苷类抗生素的生物合成

发表论文

1: Han T, Yamada-Mabuchi M, Zhao G, Li L, Liu G, Ou HY, Deng Z, Zheng Y*, He X*. Recognition and cleavage of 5-methylcytosine DNA by bacterial SRA-HNH proteins. Nucleic Acids Res. 2015 Jan; 43(2):1147-59.
2: Zhao G, Wu G, Zhang Y, Liu G, Han T, Deng Z, He X*. Structure of theN-glycosidase MilB in complex with hydroxymethyl CMP reveals its Arg23specifically recognizes the substrate and controls its entry. Nucleic Acids Res. 2014 Jul; 42(12):8115-24.
3: Liu G, Ou HY, Wang T, Li L, Tan H, Zhou X, Rajakumar K, Deng Z*, He X*. Cleavage of phosphorothioated DNA and methylated DNA by the type IV restriction endonuclease ScoMcrA. PLoS Genet. 2010 Dec 23; 6(12):e1001253.
4: Liu G, Zhang Z, Zhao G, Deng Z, Wu G*, He X*. Crystallization and preliminary X-ray analysis of the type IV restriction endonuclease ScoMcrA from Streptomyces coelicolor, which cleaves both Dcm-methylated DNA and phosphorothioated DNA. Acta Crystallogr F Struct Biol Commun. 2015 Jan 1; 71(Pt 1):57-60.
5: Feng J, Wu J, Gao J, Xia Z, Deng Z, He X*. Biosynthesis of the β-methylarginine residue of peptidyl nucleoside arginomycin in Streptomyces arginensis NRRL 15941. Appl Environ Microbiol. 2014 Aug; 80(16):5021-7. doi: 10.1128/AEM.01172-14.
6: Feng J, Wu J, Dai N, Lin S, Xu HH, Deng Z, He X*. Discovery and characterization of BlsE, a radical S-adenosyl-L-methionine decarboxylase involved in the blasticidin S biosynthetic pathway. PLoS One. 2013 Jul18; 8(7):e68545.
7: Li L, Wu J, Deng Z, Zabriskie TM, He X*. Streptomyces lividans blasticidin S deaminase and its application in engineering a blasticidin S-producing strain for ease of genetic manipulation. Appl Environ Microbiol. 2013 Apr; 79(7):2349-57.
8: Wu J, Li L, Deng Z, Zabriskie TM, He X*. Analysis of the mildiomycin biosynthesis gene cluster in Streptoverticillum remofaciens ZJU5119 and characterization of MilC, a hydroxymethyl cytosyl-glucuronic acid synthase. Chembiochem. 2012 Jul 23; 13(11):1613-21.
9: Yu Q, Du A, Liu T, Deng Z*, He X*. The biosynthesis of the polyether antibiotic nanchangmycin is controlled by two pathway-specific transcriptional activators. Arch Microbiol. 2012 Jun; 194(6):415-26.
10: Ou HY, Kuang SN, He X, Molgora BM, Ewing PJ, Deng Z, Osby M, Chen W, Xu HH*. Complete genome sequence of hypervirulent and outbreak-associated Acinetobacter baumannii strain LAC-4: epidemiology, resistance genetic determinants and potential virulence factors. Sci Rep. 2015 Mar 2; 5:8643.
11: Bi D, Xu Z, Harrison EM, Tai C, Wei Y, He X, Jia S, Deng Z, Rajakumar K, Ou HY*. ICEberg: a web-based resource for integrative and conjugative elements found in Bacteria. Nucleic Acids Res. 2012 Jan; 40(Database issue):D621-6.
12: Li J, Qu X, He X, Duan L, Wu G, Bi D, Deng Z, Liu W, Ou HY*. ThioFinder: a web-based tool for the identification of thiopeptide gene clusters in DNAsequences. PLoS One. 2012; 7(9):e45878.
13: Xu F, Kong D, He X, Zhang Z, Han M, Xie X, Wang P, Cheng H, Tao M, Zhang L, Deng Z, Lin S*. Characterization of streptonigrin biosynthesis reveals a crypticcarboxyl methylation and an unusual oxidative cleavage of a N-C bond. J Am Chem Soc. 2013 Feb 6; 135(5):1739-48.
14: Gu TP, Guo F, Yang H, Wu HP, Xu GF, Liu W, Xie ZG, Shi L, He X, Jin SG, IqbalK, Shi YG, Deng Z, Szabó PE, Pfeifer GP, Li J*, Xu GL*. The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes. Nature. 2011 Sep4; 477(7366):606-10.

研究成果

1.首次报道能够识别DNA骨架磷硫酰化修饰的IV限制酶。
2.克隆了多个六元环糖核苷类抗生素的合成基因簇。
3.参与了DNA硫修饰基因簇的克隆和DNA硫元素的发现。
4.阐明了细菌中次生代谢合成基因簇编码蛋白形成和识别5-羟甲基胞嘧啶的机制。